Liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for quantitative estimation of moxifloxacin in human plasma

doi:10.5246/jcps.2014.03.020

Journal of Chinese Pharmaceutical Sciences

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Liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for quantitative estimation of moxifloxacin in human plasma

Vipul. M. Vaghela, Prajesh Prajapati*, Hetal K. Patel

1. Shree Swaminarayan Sanskar Pharmacy College, Zundal-382421, Gujarat, India
2. A. R. College of Pharmacy and G. H. Patel Institute of Pharmacy, Vallabh Vidhyanagar-388120, Gujarat, India
3. Institute of Research and Development, Gujarat Forensic Sciences University, Gandhinagar-382007, Gujarat, India

Received:2013-10-07 Revised:2013-10-29 Online:2014-03-13 Published:2013-11-07
Contact: *Corresponding author. Tel.: +91-9825318996; Fax: 07923247465; E-mail: prajeshprajapati@gmail.com
About author:*Corresponding author. Tel.: +91-9825318996; Fax: 07923247465; E-mail: prajeshprajapati@gmail.com

Abstract

Abstract:

A rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of moxifloxacin (MOXI) in human plasma. After a simple protein precipitation using acetonitrile, the post treatment samples were analysed on a C₁₈ column interfaced with a Triple Quadropole Tandem Mass Spectrometer. Positive electrosprayionization was employed as the ionization source. The mobile phase consisted of 0.1% formic acid–acetonitrile (60:40, v/v). Ciprofloxacin (CIPRO) was used as an internal standard. The analyte and internal standard (CIPRO) were monitored in the multiple reaction monitoring mode (MRM). The mass transition ion-pair has been followed as m/z 402→358.2 for MOXI and 332→288.1 for CIPRO. The method was linear in the concentration range of 25–5000 ng/mL. The lower limit of quantification was 25 ng/mL. The intra- and inter-day precision (relative standard deviation) and accuracy (relative error) values were within 12.4%. Each plasma sample was analyzed within 3 min.

Key words: Moxifloxacin, Ciprofloxacin, Human plasma, Liquid chromatography tandem mass spectrometry, Electrospray ionization

CLC Number:

R917

Supporting:

Vipul. M. Vaghela, Prajesh Prajapati, Hetal K. Patel. Liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for quantitative estimation of moxifloxacin in human plasma[J]. Journal of Chinese Pharmaceutical Sciences, DOI: 10.5246/jcps.2014.03.020.

References

[1] Toltizis, P.; Golver, M.L.; Reed, M.D. Pharmacotherapy: A Pathophysiologic Approach (4th ed.), Appleton & Lange, Stamford, CT, 1999, 1651–1684.

[2] Petersen, U.; Dalhoff, A.; Endermann, R. Proceedings of the 36th ICAAC, vol. 100, New Orleans, LA, Poster F1, 1996.

[3] Woodcock, J.M.; Andrews, J.M.; Broswell, F.J.; Brenwald, N.P.; Wise, R. Antimicrob. Agents Chemother. 1997, 41, 101–106.

[4] Fass, R.J. Antimicrob. Agents Chemother. 1997, 41, 1818–1824.

[5] Dalhoff, A.; Peterson, U.; Endermann, R. Chemotherapy. 1997, 42, 410–425.

[6] Bruggemann, A.B.; Kugler, K.C.; Doern, G.V. Antimicrob. Agents Chemother. 1997, 41, 1594–1597.

[7] Roblin, P.M.; Hammerschlag, M.R. Antimicrob. Agents Chemother. 1998, 42, 951–952.

[8] Donati, M.; Rodriguez, F.M.; Olmo, A.; Apote, L.D.; Ceben, R. Antimicrob. Agents Chemother. 1999, 43, 825–827.

[9] AVELOX (package insert), Bayer Corporation, West Haven, CT, December. 1999.

[10] Zhao, X.; Xu, C.; Domagala, J.; Drlica, K. Proc. Natl. Acad. Sci. 1997, 94, 13991–13996.

[11] Blondeau, J.M. Clin. Ther. 1999, 21, 3–40.

[12] Biedenbach, D.L.; Barrett, M.S.; Croco, M.A.T.; Jones, R.N. Diagn. Microbiol. Infect. Dis. 1998, 32, 45–50.

[13] Poon, G. Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation and Applications, Wiley, New York, 1997, 323–324.

[14] Watson, J. Introduction to Mass Spectrometry, Lippincott-Raven, Philadelphia, 1997, 402–404.

[15] Whitehouse, C.M.; Dreyer, R.N.; Yamashita Fenn, J.B. Anal. Chem. 1985, 57, 675–679.

[16] Haniszewski, J.S.; Rogers, K.J.; Whalen, K.M.; Cole, M.J.; Liston, T.E.; Duchoslav, E.; Fouda, H.G. Anal. Chem. 2001, 73, 1495–1501.

[17] Korfmacher, W.A.; Cox, K.A.; Ng, K.J.; Veals, J.; Hsieh, Y.; Wainhaus, S.; Broske, L.; Prelusky, D.; Nomier, A.; White, R.E. Rapid Commun. Mass Spectrosc. 2001, 15, 335–340.

[18] Tobin, C.M.; Sunderland, J.; White, L.O.; MacGowan, A.P.; Reeves, D.S. Antimicrob. Agents Chemother. 1998, 42, 278–279.

[19] Stass, H.; Dalhoff, A. J. Chromatogr. B. 1997, 702, 163–174.

[20] Lemoine, T.; Breilh, D.; Ducint, D.; Dubrez, J.; Jougon, J.; Valley, J.F.; Saux, M.C. J. Chromatogr. B. 2000, 742, 247–254.

[21] Ba, B.B.; Etienne, R.; Ducint, D.; Quentin, C.; Saux, M.C. J. Chromatogr. B. 2001, 754, 107–112.

[22] Moller, J.G.; Stass, H.; Heining, R.; Blaschke, G. J. Chromatogr. B. 1998, 716, 325–334.

[23] Ocana, J.A.; Barragan, F.J.; Callejon, M. Analyst. 2000, 125, 2322–2325.

[24] Vishwanathan, K.; Barlett, M.G.; Stewart, J.T. J. Pharm. Biol. Anal. 2002, 30, 961–968.

Liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for quantitative estimation of moxifloxacin in human plasma

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