A cell-combining-oriented phytochemical screening strategy using triple quadrupole mass spectrometry for the investigation of potential bioactive candidates in Chinese traditional medicine preparation Xiao-ai-ping injection

doi:10.5246/jcps.2023.02.008

Abstract

Abstract:

Xiao-ai-ping injection (XAP-I), obtained from the extract of Marsdenia tenacissima stem, is widely used for the treatment of various cancers in China, and the suitable methods to elucidate bioactive compounds from the complex mixtures of XAP-I have stirred up great enthusiasm for many investigators. In the present study, we identified the major pregnane oligoglycoside and phenolic acid components of XAP-I using an improved triple quadrupole mass spectrometry (QqQ-MS) detection. Moreover, a combination of full scan (FS), precursor ion scan (PCIS), product ion scan (PDIS), and multiple reaction monitoring (MRM) modes could take advantage of extensive adduct formation to analyze the identity of ingredients and provide the ingredient profiling in XAP-I with high sensitivity. Furthermore, the cell-combining-oriented phytochemical screening strategy was used to screen bioactive ingredients of XAP-I. This strategy could achieve a high-throughput qualitative and quantitative analysis of XAP-I for screening potential bioactive candidates of XAP-I sensitively and selectively.

Key words: Xiao-ai-ping injection, Triple quadrupole mass spectrometry, Screening, Bioactive candidates, HepaRG cells

Supporting: /attached/file/20230302/20230302162500_130.pdf

Zhi Rao, Yuhui Wei. A cell-combining-oriented phytochemical screening strategy using triple quadrupole mass spectrometry for the investigation of potential bioactive candidates in Chinese traditional medicine preparation Xiao-ai-ping injection[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(2): 101-111.

Figures/Tables 8

Table 1. The information for MRM parameters used for characterization of phenolic acids in XAP-I.

Table 2. The bioactive candidates with HepaRG cells in XAP-I.

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