Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV

doi:10.5246/jcps.2014.03.022

Journal of Chinese Pharmaceutical Sciences

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Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV

Qingrong Fu, Shujuan Liu, Hong Wang, Shizhong Chen^*

Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2013-07-24 Revised:2013-10-13 Online:2014-03-13 Published:2013-10-28
Contact: *Corresponding author. Tel.: 86-10-82802723; Fax: 86-10-82802723; E-mail: chenshizhong66@163.com
About author:*Corresponding author. Tel.: 86-10-82802723; Fax: 86-10-82802723; E-mail: chenshizhong66@163.com
Supported by:
Study of Nature of Geo-authentic Crude Drug (“973” State Key Project, Grant No. 2006CB504700).

Abstract

Abstract:

A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricitrin, hyperoside, galuteolin, avicularin, quercitrin, quercetin, luteolin, and kaempferol. The analysis was performed on an Inertsil ODS-4 column (4.6 mm×150 mm, 5 µm) with gradient elution. The mobile phases were 0.5% aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The eight marker flavonoids were separated well with good linearity (r²>0.9991), precision, stability and repeatability. The recovery rate was 95.58%–102.65%. Cluster analysis was employed to analyze 28 batches of samples. The result indicated that this method provides an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of Polygonum aviculare L.

Key words: Polygonum aviculare L., Flavonoids, Quantitative analyses, Cluster analysis

CLC Number:

R284.1

Supporting:

Qingrong Fu, Shujuan Liu, Hong Wang, Shizhong Chen. Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV[J]. Journal of Chinese Pharmaceutical Sciences, DOI: 10.5246/jcps.2014.03.022.

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Simultaneous determination of eight flavonoids in Polygonum aviculare L. by RP-HPLC-UV

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