Simultaneous quantification of eight active compounds in Sinopodophyllum hexandrum by HPLC-DAD

doi:10.5246/jcps.2015.06.048

Abstract

Abstract:

Sinopodophylli Fructus is the commonly used traditional Tibetan medicinal herb. In the present study, we established a reversed-phase high performance liquid chromatography method to simultaneously determine three lignans and five flavonoid constituents, namely podophyllotoxin, desoxypodophyllotoxin, 4′-demethyldesoxypodophyllotoxin, 8-prenylkaemferol, quercetin, kaempferol, 8,2'-diprenylquercetin 3-methylether and 8-prenylquercetin, in Sinopodophylli Fructus. The chromatographic separationwas achieved on a C₁₈analytical column with a gradient mobile phase consisting of acetonitrile and 0.05% phosphoric acid at a flowrate of 1.0 mL/min. UV detection was set at 290 nm and 370 nm, and the column oven was set at 35 °C. This method provided a good reproducibility, and its overall intra- and inter-day precision was less than 3% and 4%, respectively. The recovery of the method was 98.29%–101.60%, and a good linearity (R²≥0.9992) was obtained for all the analytes over a relatively wide range of concentration. A total of 17 samples of S. hexandrum (12 fruits, 5 roots and rhizomes) were collected from different areas and then successfully quantified. The results indicated that the contents of eight compounds significantly varied (the sum content rangedfrom 16.90 to 55.68 mg/g), and prenylated flavonoids could be used as marker constituents in the identification and quality control of Sinopodophylli Fructus.

Key words: Sinopodophyllum hexandrum, HPLC, Flavonoids, Lignans, Quality control

CLC Number:

R284

Supporting:

Aihua Wang, Yue Kong, Mingying Shang, Rongyun You, Guangxue Liu, Feng Xu, Shaoqing Cai. Simultaneous quantification of eight active compounds in Sinopodophyllum hexandrum by HPLC-DAD[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(6): 376-382.

References

[1] China Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Vol. I, Beijing: China Medical Science and Technology Press. 2010, 43.

[2] Chengdu Branch, Chinese Academy of Sciences. Sichuan Chinese Medicine Record. Chengdu: Sichuan People’s Publishing House, 1962, 10.

[3] Shang, M.Y.; Xu, G.J.; Xu, L.S.; Li, P. Chin. J. Chin. Mat. Med. 1994, 19, 451-454.

[4] Nanjing University of Chinese Medicine. Zhong Yao Da Ci Dian (The Dictionary of Chinese Herbal Medicine) (2nd ed.). Shanghai: Shanghai Scientific Technical Pubnishers. 2006, 2548.

[5] Shang, M.Y.; Li, J.; Cai, S.Q.; Li, P.; Xu. L.S.; Xu, G.J. Chin. Tradit. Herb. Drugs. 2000, 31, 569-571.

[6] Kong, Y.; Xiao, J.J.; Meng, S.C.; Dong, X.M.; Ge, Y.W.; Wang, R.F.; Shang, M.Y.; Cai, S.Q. Fitoterapia. 2010, 81, 367-370.

[7] Shang, M.Y.; Wang, Q.H.; Xiao, J.J.; Shang, Y.H.; Kong, Y.; Cai, S.Q. CN 102335165A, 2012.

[8] Xu, H.; Lv, M.; Tian, X. Curr. Med. Chem. 2009, 16, 327-349.

[9] Woo, E.R.; Kwak, J.H.; Kim, H.J.; Park, H. J. Nat. Prod. 1998, 61, 1552-1554.

[10] Meragelman, K.M.; McKee, T.C.; Boyd, M.R. J. Nat. Prod. 2001, 64, 546-548.

[11] Botta, B.; Vitali, A.; Menendez, P.; Misiti, D. Curr. Med. Chem. 2005, 12, 713-739.

[12] Chi, Y.S.; Jong, H.G.; Son, K.H.; Chang, H.W.; Kang, S.S.; Kim, H.P. Biochem. Pharmacol. 2001, 62, 1185-1191.

[13] Shukla, S.K.; Chaudhary, P.; Kumara, I.P.; Afrin, F.; Puri, S.C.; Qazi, G.N.; Sharma, R.K. Environ. Toxicol. Pharm. 2006, 22, 113-120.

[14] Wang, A.H.; Liu, G.X.; Xu, F.; Shang, M.Y.; Cai, S.Q. J. Chin. Mat. Med. 2013, 38, 3528-3533.

[15] Shang, M.Y.; Kong, Y.; Xiao, J.J.; Ma, X.J.; Ge, Y.W.; Cai, S.Q. CN 101647842A, 2010.

[16] Guo, S.; Wang, L.; Su, D.; Kong, Y.; Shang, M.Y.; Cai, S.Q. J. China Pharma. 2014, 25, 577-580.

[17] Shang, M.Y.; Kong, Y.; Xiao, J.J.; Ma, X.J.; Ge, Y.W.; Cai, S.Q. CN 101648934A, 2010.

[18] Shang, M.Y.; Wang, Q.H.; Xiao, J.J.; Shang, Y.H.; Kong, Y.; Cai, S.Q. CN 102382092A, 2012.

[19] Shang, M.Y.; Xu, L.S.; Wang, Y.X.; Cai, S.Q. Chin. Tradit. Herb. Drugs. 2002, 32, 722-724.

[20] Chen, Y.; De, J.; Liu, Y.H.; Liu, Q.L.; Huang, Z.F.; Liu, Y. Chin. Tradit. Pat. Med. 2010, 32, 708-711.

[21] Chen, Y.; De, J.; Liu, Q.L.; Liu, Y.H.; Yi, J.H. West China J. Pharm. Sci. 2009, 24, 93-94.

[22] Shang, M.Y.; Xu, G.J.; Xu, L.S.; Li, P. J. Chin. Pharm. Univ. 1996, 27, 219-222.

[23] Liang, X.M.; Jin, Y.; Wang, Y.P.; Jin, G.W.; Fu, Q.; Xiao, Y.S. J. Chromatogr. A. 2009, 1216, 2033-2044.

[24] Paoletti, T.; Fallarini, S.; Gugliesi, F.; Minassi, A.; Appendino, G.; Lombardi, G. Eur. J. Pharmacol. 2009, 620, 120-130.