Quality evaluation of Farfarae Flos by ITS/ITS2 and HPLC fingerprint analysis

doi:10.5246/jcps.2021.01.006

Abstract

Abstract:

The flower bud of Tussilago farfara L., also known as Farfarae Flos (FF), is commonly used in the traditional Chinese medicine (TCM) for the treatment of cough, bronchitis and asthmatic disorders. In this study, 26 samples from five provinces across northern China were collected, and internal transcribed spacer-polymerase chain reaction (ITS-PCR) coupled with high performance liquid chromatography (HPLC) fingerprint profiling was used for the quality evaluation of FF. The results of the ITS sequence analysis showed the high similarity values among these samples. ITS2 sequence exhibited less molecular diversity compared with ITS1, and no obvious correlation was found between the variation of ITS with the production areas or cultivation methods. The results of HPLC fingerprints in combination with the similarity analysis suggested that relative contents of phenylpropanoids and flavonoids varied among the 26 FF samples, and there was also no obvious difference among the different habitats. The phenylpropanoids and the flavonoids showed similar fluctuating patterns, and positive correlation among them was also observed. The results presented in this study suggested that FF from different habits showed high similarities, and ITS-PCR coupled with HPLC fingerprint profiling could be used as a valuable approach for the quality evaluation of FF.

Key words: Farfarae Flos, ITS, ITS2, HPLC fingerprints, Multivariate analysis

Supporting:

Zijuan Lu, Yan Jia, Jie Xing, Shuxian Xiao, Yulong Wang, Wenjie Qin, Xuemei Qin, Zhenyu Li. Quality evaluation of Farfarae Flos by ITS/ITS2 and HPLC fingerprint analysis[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(1): 58-68.

Figures/Tables 6

References 22

[1]	Gao, H.; Huang, Y.N.; Gao, B.; Xu, P.Y.; Inagaki, C.; Kawabata, J. α-Glucosidase inhibitory effect by the flower buds of Tussilago farfara L. Food Chem. 2008, 106, 1195–1201.
[2]	Wu, D.; Zhang, M.; Zhang, C.F.; Wang, Z.T. Flavonoids and phenolic acid derivatives from flos farfarae. China J. Chin. Mater. Med. 2010, 35, 1142–1144.
[3]	Liu, Y.F.; Yang, X.W.; Wu, B. Studies on chemical constituents in the buds of Tussilago farfara. China J. Chin. Mater. Med. 2007, 32, 2378–2381.
[4]	Yaoita, Y.; Suzuki, N.; Kikuchi, M. Structures of new sesquiterpenoids from Farfarae Flos. Chem. Pharm. Bull. 2001, 49, 645–648.
[5]	Kong, W.J.; Zhao, Y.L.; Xiao, X.H.; Jin, C.; Li, Z.L. Quantitative and chemical fingerprint analysis for quality control of Rhizoma Coptidischinensis based on UPLC-PAD combined with chemometrics methods. Phytomedicine. 2009, 16, 950–959.
[6]	Li, Z.Y.; Zhi, H.J.; Zhang, F.S.; Sun, H.F.; Zhang, L.Z.; Jia, J.P.; Xing, J.; Qin, X.M. Metabolomic profiling of the antitussive and expectorant plant Tussilago farfara L. by nuclear magnetic resonance spectroscopy and multivariate data analysis. J. Pharm. Biomed. Anal. 2013, 75, 158–164.
[7]	Li, D.Z.; Gao, L.M.; Li, H.T.; Wang, H.; Ge, X.J.; Liu, J.Q.; Chen, Z.D.; Zhou, S.L.; Chen, S.L.; Yang, J.B.; Fu, C.X.; Zeng, C.X.; Yan, H.F.; Zhu, Y.J.; Sun, Y.S.; Chen, S.Y.; Zhao, L.; Wang, K.; Yang, T.; Duan, G.W. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. Proc. Natl. Acad. Sci. USA. 2011, 108, 19641–19646.
[8]	Luo, K.; Chen, S.L.; Chen, K.L.; Song, J.Y.; Yao, H.; Ma, X.Y.; Zhu, Y.J.; Pang, X.H.; Yu, H.; Li, X.W.; Liu, Z. Assessment of candidate plant DNA barcodes using the Rutaceae family. Sci. China Life Sci. 2010, 53, 701–708.
[9]	Selvaraj, D.; Sarma, R.K.; Shanmughanandhan, D.; Srinivasan, R.; Ramalingam, S. Evaluation of DNA barcode candidates for the discrimination of the large plant family Apocynaceae. Plant Syst. Evol. 2015, 301, 1263–1273.
[10]	Chen, X.C.; Liao, B.S.; Song, J.Y.; Pang, X.H.; Han, J.P.; Chen, S.L. A fast SNP identification and analysis of intraspecific variation in the medicinal Panax species based on DNA barcoding. Gene. 2013, 530, 39–43.
[11]	Abu Almakarem, A.S.; Heilman, K.L.; Conger, H.L.; Shtarkman, Y.M.; Rogers, S.O. Extraction of DNA from plant and fungus tissues in situ. BMC Res. Notes. 2012, 5, 1–11.
[12]	Qiao, S.L. Studies on similarity evaluation of chromatographic fingerprint for TCM. Second Military Med. Univ. 2014.
[13]	Zhang, Z.P.; Zhang, Y.; Zhang, Z.; Yao, H.; Liu, H.T.; Zhang, B.G.; Liao, Y.H. Comparative analysis of DNA barcoding and HPLC fingerprint to trace species of phellodendri cortex, an important traditional Chinese medicine from multiple sources. Biol. Pharm. Bull. 2016, 39, 1325–1330.
[14]	Li, D.Z.; Liu, J.Q.; Chen, Z.D.; Wang, H.; Ge, X.J.; Zhou, S.L.; Gao, L.M.; Fu, C.X.; Chen, S.L. Plant dna barcoding in China. J. Syst. Evol. 2011, 49, 165–168.
[15]	Yang, P.; Li, X.W.; Zhou, H.; Hu, H.; Zhang, H.; Sun, W.; Wang, Y.T.; Yao, H. Molecular identification of Chinese materia medica and its adulterants using ITS2 and psbA-trnH barcodes: a case study on rhizoma menispermi. Chin. Med. 2014, 5, 190–198.
[16]	Yu, H.; Wu, K.Y.; Song, J.Y.; Zhu, Y.J.; Yao, H.; Luo, K.; Dai, Y.; Xu, S.J.; Lin, Y.L. Expedient identification of Magnoliaceae species by DNA barcoding. Plant Omics. 2014, 7, 47–53.
[17]	Ma, X.Y.; Xie, C.X.; Liu, C.; Song, J.Y.; Yao, H.; Luo, K.; Zhu, Y.J.; Gao, T.; Pang, X.H.; Qian, J.; Chen, S.L. Species identification of medicinal pteridophytes by a DNA barcode marker, the chloroplast psbA-trnH intergenic region. Biol. Pharm. Bull. 2010, 33, 1919–1924.
[18]	Deng, W.P.; Xu, T.T.; Yang, M.; Cui, Y.J.; Guo, D.A. Improved chromatographic fingerprinting combined with multi-components quantitative analysis for quality evaluation of Penthorum chinense by UHPLC-DAD. Nat. Prod. Commun. 2015, 10, 71–76.
[19]	Zhang, W.; Yang, T.S.; Wang, X.P.; Dang, W.X. HPLC determination of chlorogenic acid and rutin contents in Flos Farfarae. Chin. J. Pharm. Anal. 2008, 28, 2106–2108.
[20]	Viapiana, A.; Wesolowski, M. HPLC fingerprint combined with quantitation of phenolic compounds and chemometrics as an efficient strategy for quality consistency evaluation of sambucus nigra berries. Nat. Prod. Commun. 2016, 11, 1449-1454.
[21]	Goodarzi, M.; Russell, P.J.; Vander Heyden, Y. Similarity analyses of chromatographic herbal fingerprints: a review. Anal. Chimica Acta. 2013, 804, 16–28.
[22]	Yudthavorasit, S.; Wongravee, K.; Leepipatpiboon, N. Characteristic fingerprint based on gingerol derivative analysis for discrimination of ginger (Zingiber officinale) according to geographical origin using HPLC-DAD combined with chemometrics. Food Chem. 2014, 158, 101–111.