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Fingerprint analysis of different Panax herbal species by HPLC-UV method

Feng Zeng, Xiao-Ming Wang, Min Yang, Zhi-Qiang Lu, De-An Guo*   

  1. 1. School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
    2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • Received:2007-04-24 Revised:2007-11-10 Online:2007-12-15 Published:2007-12-15
  • Contact: De-An Guo*

Abstract:

Aim To establish a method for differentiating commercial samples of Panax species including notoginseng, cultivated ginseng (Chinese ginseng and Korean ginseng), wild ginseng, red ginseng, three types of American ginsengs, and one American ginseng preparation with their HPLC fingerprints for assnrning the quality of different commercial samples of Panax species. Methods HPLC-UV method was used to establish their fingerprints, Zorbax Extend C18 (250 mm × 4.6 mm, 5 μm) was used as the analytical column, and acetonitrile/KH2PO4 aqueous solution was used as the mobile phase with gradient elution. Results The fingerprints of different commercial samples of Panax species varied in their holistic chromatograms and some specific constituents. Conclusion This method is reliable, reproducible and simple. It could be applied in the routine authentication of different commercial samples of Panax species.

Key words: Ginseng, Ginseng, Panax species, Panax species, HPLC fingerprint, HPLC fingerprint

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