Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint

doi:10.5246/jcps.2015.10.082

Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (10): 647-653.DOI: 10.5246/jcps.2015.10.082

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Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint

Zhucan Lin¹, Long Zhang¹, Ruizhuo Zhang¹, Anyu Huang¹, Suhua Guo^1,2*

1. State Key Laboratory of Chinese Pharmacies, Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2. Fujian Health College, Fuzhou 350101, China

Received:2015-05-08 Revised:2015-05-25 Online:2015-10-21 Published:2015-06-10
Contact: Tel.: 86-591-22861135, E-mail: guosuhua2005@126.com
Supported by:
Quality guarantee system of Chinese herbal medicines (Grant No. 201507002), Key project of science and technology of Fujian province (Grant No. 2014Y0053) and youth project of Fujian Provincial Health and Family Planning Commission (Grant No. 2015-1-77).

Abstract

Abstract:

Sedum aizoon L. (SA) has been widely used for treatment of various hemorrhages, insomnia, pain and trauma; however, its anti-inflammatory activity is unknown. In this study, wefirstly investigated the anti-inflammatory activity of SA extracts by petroleum ether (PE), ethyl acetate (EtOAc), and H₂O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF-α, and IL-6 production in RAW 264.7 cells (P<0.01), suggesting that this extract possessed potent anti-inflammatoryactivity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p-hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the EtOAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti-inflammatory agent.

Key words: Sedum aizoon L., EtOAc extract, RAW 264.7 cell, Pro-inflammatory cytokines and mediators, HPLC fingerprint

CLC Number:

R931.5

Supporting:

Zhucan Lin, Long Zhang, Ruizhuo Zhang, Anyu Huang, Suhua Guo. Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(10): 647-653.

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Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint

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