Development of the fingerprints of crude Pu-erh tea and ripened Pu-erh tea by high­performance liquid chromatography

doi:10.5246/jcps.2011.04.044

Abstract

Abstract:

A simple and accurate high-performance liquid chromatography (HPLC) method to generate the fingerprints of crude Pu-erh tea (CPT) and ripened Pu-erh tea (RPT) is described. A suitable chromatographic system was established using a linear gradient elution with acetonitrile and water containing 0.6% formic acid as the mobile phase and a detection wavelength of 300 nm. HPLC analysis identified 24 common peaks among RPT and 21 common peaks among CPT. This study revealed that crude Pu-erh tea and ripened Pu-erh tea contained some similar major constituents, but with distinctive peaks, which may be caused by the difference in the fermentation process. This HPLC fingerprint method could be used to evaluate and authenticate crude Pu-erh tea and ripened Pu-erh tea.

Key words: HPLC fingerprint, Pu-erh tea, Quality control

CLC Number:

R284.1

Supporting:

Liang Zhang, Ning Li, Yan-Yun Che, Zhi-Zhong Ma, Peng-Fei Tu^*. Development of the fingerprints of crude Pu-erh tea and ripened Pu-erh tea by highperformance liquid chromatography [J]. , DOI: 10.5246/jcps.2011.04.044.

[1]	Shi Jin, Chengyin Li, Xinyue Jing, Yunru Peng, Shaohua Dong, Wanjin Sun. Study on quality control of Xiaozhong Ointment NO. 1 based on the relationship between quality fractions of main components in monarch and minister drugs and the clinical efficacy [J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(9): 764-771.
[2]	Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan. An overview of Pu-erh tea and its health-promoting effects of lipid-lowering and anti-obesity [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(8): 623-633.
[3]	Yizi Zhu, Huajun Li, Yubo Zhang, Chaoran Zhao, Shuai Lu, Mingxuan Fan, Mei Han. Comparison of quality control standards of PET radiopharmaceuticals for tumor in Pharmacopoeia of Europe, the United States and People’s Republic of China [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(3): 246-252.
[4]	Zijuan Lu, Yan Jia, Jie Xing, Shuxian Xiao, Yulong Wang, Wenjie Qin, Xuemei Qin, Zhenyu Li. Quality evaluation of Farfarae Flos by ITS/ITS2 and HPLC fingerprint analysis [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(1): 58-68.
[5]	Hongshuai Yang, Qiuyi Liu, Weidong Yin, Manjiang Xie, Xiang Ji, Shuangyu Shao, Hong Liang, Pengfei Tu, Qingying Zhang. Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(4): 252-259.
[6]	Jinhui Shi, Ruiyue Li, Siyu Yang, Hongmei Zhang. Phytochemistry, biological properties and quality control of Chuanxiong Rhizoma: a review [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(11): 755-779.
[7]	Rengeng Shu, Xiluan Yan, Shasha Li, Pengfei Tu. Simultaneous determination of l-calycanthine, quercetin, and kaempferol in Chimonanthi Nitentis Folium by RP-HPLC [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(11): 805-810.
[8]	Jing Ye, Wenguang Wang, Jun Li, Xiaoyu Guo, Mingbo Zhao, Yong Jiang, Pengfei Tu. Influence of the post-fermentation by four Aspergillus strains on the aroma of pu-erh tea [J]. Journal of Chinese Pharmaceutical Sciences, 2016, 25(4): 284-290.
[9]	Aihua Wang, Yue Kong, Mingying Shang, Rongyun You, Guangxue Liu, Feng Xu, Shaoqing Cai. Simultaneous quantification of eight active compounds in Sinopodophyllum hexandrum by HPLC-DAD [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(6): 376-382.
[10]	Li Pan, Xi Wen, Yu Sha, Wenting Zhu, Maosheng Cheng. Synthesis of impurity B of Flumazenil [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(2): 133-136.
[11]	Zhucan Lin, Long Zhang, Ruizhuo Zhang, Anyu Huang, Suhua Guo. Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(10): 647-653.
[12]	Yi Liu, Wei Wang, Yuying Zhao, Hubiao Chen, Hong Liang, Qingying Zhang. Chemical analysis of the Hedysarum multijugum root by HPLC fingerprinting [J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(10): 654-659.
[13]	Jinfeng Chen, Pengfei Tu, Yong Jiang. HPLC fingerprint-oriented preparative separation of major flavonoids from safflower extract by preparative pressurized liquid chromatography [J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(7): 490-495.
[14]	Yan Zhao, Min Liu, Zhao Yang, Shaohua Wang, Yang Li, Guanhua Du. Simultaneous quantification of eighteen constituents in traditional Chinese medicine XiaoShuanTongLuo by UPLC-MS/MS [J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(4): 225-232.
[15]	Zhiyan Lin, Rongfu Yang, Yuenian Tang, Huaiping Tian, Jian Zhang. Application of UHPLC-MS/MS method in determining the content of multi-ingredient in hospital preparations [J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(4): 233-240.