Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray Mass Spectrometry

Abstract

Abstract: Aim To develop a HPLC-UV-MS method for identifying the constituents in the Chinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C18 column (250 mm×4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrile containing 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range 195-400 nm. Both positive and negative ion ESI modes were used. Results The constituents in Notoginseng were well separated and detected. Fourteen compounds were identified by comparing their retention time and ESI-MS data with those obtained from the reference compounds. Forty-one compounds were deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E, chikusetsusaponin-L5, malonyl-ginsenoside-Rg1, the isomers of notoginsenosides-J, -A, -R1, -G, -R2, and ginsenoside-Rh3 were discovered in Notoginseng for the first time. Conclusion This method gives high sensitivity and good separation, and is suitable for identifying the constituents in Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based on this result, further quality control can be studied.

Key words: Panax notoginseng, Panax notoginseng, HPLC-MS, HPLC-MS, ginsenosides, ginsenosides, notoginsenosides, notoginsenosides, flavonoids, flavonoids

CLC Number:

R284.1

R917.01

Supporting:

LIU Jin-huai, WANG Xuan^*, CAI Shao-qing, KOMATSU Katsuko, NAMBA Tsuneo. Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray Mass Spectrometry[J]. .

References

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