Development of an HPLC-UV method for the determination of ethaselen in its injection formulation

doi:10.5246/jcps.2012.04.043

Abstract

Abstract:

A novel ethaselen injection formulation has been developed in our laboratory. The objective of the present study was to establish and validate a high performance liquid chromatography (HPLC) method for the determination of ethaselen in its injection formulation. Analysis was performed on an ODS column with isocratic elution at 40 °C. Mobile phase was consisted of 0.01% phosphoric acid and methanol (60:40, v/v). The detection wavelength was set at 320 nm and the flow rate was 1.0 mL/min. The results showed that the calibration curves of ethaselen were linear in the range of 10-50 µg/mL (r² = 0.9999). The limit of detection for ethaselen was 100 ng/mL. The average recovery of ethaselen was 100.14%. The labeled content of ethaselen in its injection formulation was in the range of 102%-103% of the measured content. In conclusion, this method was stable and reproducible, thus providing a useful tool for the routine analysis of this novel ethaselen injection formulation.

Key words: Ethaselen, Injection, HPLC, Content

CLC Number:

R917.4

Supporting:

Su Chen, Ping Song, Ying Wang, Qi-Zhi Hu, Xuan Zhang^*, Qiang Zhang. Development of an HPLC-UV method for the determination of ethaselen in its injection formulation[J]. , DOI: 10.5246/jcps.2012.04.043.

[1]	Weiwei Xie, Jian Liu, Yuqian Zhang, Zhiqing Zhang, Yiran Jin. A case of anaphylactic shock caused by iodixanol during transjugular intrahepatic portosystemic shunt and literature analysis [J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(8): 677-682.
[2]	Zhi Rao, Yuhui Wei. A cell-combining-oriented phytochemical screening strategy using triple quadrupole mass spectrometry for the investigation of potential bioactive candidates in Chinese traditional medicine preparation Xiao-ai-ping injection [J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(2): 101-111.
[3]	Yanqing Zhou, Yonghong Liu, Yanting Li, Yanru Tao, Jin Li, Xinhui Jiang. Determination of chlorogenic acid and rutin in extractive solution of ficus virens leaves by HPLC-PDA [J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(1): 45-51.
[4]	Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan. Matrine, oxymatrine, and compound Kushen injection from the roots of Sophora flavescens: an overview of their anticancer activities [J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(5): 321-333.
[5]	Xiangfei Jin, Minyue Cheng, Sihui Zhong, Yungen Xu, Weiyang Shen. Identification of unknown impurities in triamcinolone acetonide palmitate [J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(4): 270-278.
[6]	Zipeng Qiao, Qizhi Wang, Jianfu Liu, Mingyuan Wang. Antioxidant and antibacterial activities of the ethyl acetate extracts of endophytic fungi isolated from Ageratum conyzoides L. [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(5): 421-433.
[7]	Xin Dong, Hong Wang, Feixiang Ma, Jianping Gao, Shizhong Chen, Peifeng Xue. A strategy for structure-activity relationship study on antioxidants in Echinops latifolius Tausch extracts by online HPLC-radical scavenging detection coupled with ESI-IT-TOF-MSn [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(4): 267-279.
[8]	Xiaojiao Li, Shuxin Luan, Hong Zhang, Hongquan Wan, Hong Chen, Chengjiao Liu, Chang Liu, Yanhua Ding. Pharmacokinetics, pharmacodynamics and safety of a single-dose long-acting Risperidone injection in Chinese patients with schizophrenia [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(3): 206-217.
[9]	Zijuan Lu, Yan Jia, Jie Xing, Shuxian Xiao, Yulong Wang, Wenjie Qin, Xuemei Qin, Zhenyu Li. Quality evaluation of Farfarae Flos by ITS/ITS2 and HPLC fingerprint analysis [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(1): 58-68.
[10]	Yi Zhou, Junli Lai, Feng Qiu, Jun Zhang. Development and validation of an HPLC-UV method for routine trough plasma concentration monitoring of imatinib in Chinese patients with gastrointestinal stromal tumor [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(9): 637-648.
[11]	Shaojing Liu, Bei Qin, Hongfang Han, Li Li, Lili Yu, Xiaojing Xu. Preparative separation of high-purity troxerutin and related substances from mother liquor of troxerutin by silica gel column chromatography and semi-preparative liquid chromatography [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(7): 487-493.
[12]	Hongshuai Yang, Qiuyi Liu, Weidong Yin, Manjiang Xie, Xiang Ji, Shuangyu Shao, Hong Liang, Pengfei Tu, Qingying Zhang. Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(4): 252-259.
[13]	Fei Yu, Wenli Zhou, Jiayi Kan, Can Peng. Comparison of dissolution profile characteristics of 11 berberine hydrochloride tablet brands in different dissolution media [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(2): 102-112.
[14]	Weijuan Jia, Shizhong Chen, Jianhua Sun, Zongli Bai, Yazhuo Huang, Yuankuan Zhang, Hong Wang. Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(12): 896-907.
[15]	Lulu Xu, Zhanpeng Shang, Yue Chai, Yang Zhang, Rong Yu, Min Ye, Xue Qiao. Detection of minor constituents in TongMai Granules using NP×RP off-line two-dimensional liquid chromatography coupled with orbitrap mass spectrometry [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(11): 793-803.

Development of an HPLC-UV method for the determination of ethaselen in its injection formulation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments