A pharmacokinetic study of puerarin formulated in mixed micelles in Beagle dogs by HPLC

doi:10.5246/jcps.2012.04.044

Abstract

Abstract:

We developed an HPLC method to study the pharmacokinetics of puerarin in Beagle dogs after oral administration of puerarin or puerarin mixed micelles (PMMS). Beagle dogs were divided into two groups randomly, and the blood samples were collected at fixed time intervals after single oral administration of puerarin or PMMS at a dosage of 120 mg/kg. Following sample preparation, analytes were separated on a C18 column (DIKMA, 150 mm×4.6 mm, 5 μm) with a guard column (DIKMA, 8 mm×4 mm) and eluted with methanol-water (25:75, v/v). Theophylline was used as the internal standard. WinNonlin 6.1 (Pharsight, USA) was used to calculate the pharmacokinetic parameters. The pharmacokinetic parameters for puerarin and PMMS in Beagle dogs were as follows: AUC, 515.96 and 2796.43 min·µg/mL; Tmax, 61.48 and 202.91 min; CL/F, 232.58 and 42.91 mL/min/kg, respectively. The relative bioavailability of PMMS to puerarin was 542.0%. Our results showed that the mixed micelle preparation significantly improved the bioavailability of puerarin by delaying absorption and reducing clearance.

Key words: Puerarin, Mixed micelles, Bioavailability, Beagle dogs, Pharmacokinetics

CLC Number:

R969.1

Supporting:

Yan-Jiao Li, Hui-Min Liu, Xiao-Fang Dang, Shuo-Feng Zhang, Jin-Ying Wu, Zhan-Hong Jia, Li-Wei Han^*. A pharmacokinetic study of puerarin formulated in mixed micelles in Beagle dogs by HPLC[J]. , DOI: 10.5246/jcps.2012.04.044.

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A pharmacokinetic study of puerarin formulated in mixed micelles in Beagle dogs by HPLC

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