Determination of luteolin and acteoside in Siphonostegiae Herba by high-performance liquid chromatography

doi:10.5246/jcps.2012.04.045

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Determination of luteolin and acteoside in Siphonostegiae Herba by
high-performance liquid chromatography

Ming-Bo Zhao, He-Lin Wei, Jun Li, Peng-Fei Tu^*

1. State Key Laboratory of Natural and Biomimetic Drugs; Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China

Received:2012-03-09 Revised:2012-05-20 Online:2012-06-25 Published:2012-06-25
Contact: Peng-Fei Tu*

Abstract

Abstract:

A sensitive RP-HPLC-DAD method has been developed and validated for the determination of luteolin and acteoside in the herb of Siphonostegia chinensis Benth. (Siphonostegiae Herba). Separation was achieved on an Agilent Zorbax SB-Aq C18 column (250 mm×4.6 mm, 5 μm) using a gradient elution with mobile phases of 0.05% phosphoric acid aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1.0 mL/min with detection at 310 nm and 350 nm. Luteolin and acteoside showed good linearity in the ranges of 0.0341-0.8172 mg/mL (r² = 0.9999) and 0.0708-2.832 mg/mL (r² = 0.9999) with average recoveries of 102.7% and 98.3%, respectively. The contents of luteolin and acteoside varied greatly in 15 samples from different habitats. This is the first report on the quantitative determination of acteoside in Siphonostegiae Herba.

Key words: Siphonostegiae Herba, Siphonostegia chinensis Benth., Luteolin, Acteoside, HPLC-DAD

CLC Number:

R931

Supporting:

Ming-Bo Zhao, He-Lin Wei, Jun Li, Peng-Fei Tu^*. Determination of luteolin and acteoside in Siphonostegiae Herba by
high-performance liquid chromatography[J]. , DOI: 10.5246/jcps.2012.04.045.

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Determination of luteolin and acteoside in Siphonostegiae Herba by
high-performance liquid chromatography

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