Simultaneous analysis of 12 bioactive constituents in Gegen Qinlian pill by HPLC-DAD

doi:10.5246/jcps.2010.06.062

Abstract

Abstract:

In this study, a novel and simple high performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous qualitative and quantitative determination of 12 bioactive components in Gegen Qinlian pill was developed. The separation was performed on a Kromasil C18 column (250 mm×4.6 mm, 5.0 μm) by gradient elution with acetonitrile and 0.02 mol/L ammonium acetate (containing 0.3% triethylamine, and adjusted pH to 4.3 using 1% glacial acetic acid) as the mobile phase at a flow rate of 0.7 mL/min. Three different detection wavelengths (250, 280, 346 nm) were set at the maximum UV absorption wavelengths of these components. Twelve components (puerarin, daidzin, baicalin, wogonoside, liquiritin, berberine, palmatine, jatrorrhizine, glycyrrhizin, baicalein, wogonin and daidzein) were identified and determined using the developed method. All calibration curves showed good linear regression (r>0.9995) within tested ranges. The injection precision, intra-day precisions and analysis repeatability were evaluated with the RSD values, which were no more than 0.97%, 1.69% and 1.71%, respectively. The recoveries were ranged from 97.4% to 100.2% with RSD values less than 1.87%. This readily available, low-cost and reliable HPLC-DAD method would improve the quality control of Gegen Qinlian pill.

Key words: Simultaneous analysis, Gegen Qinlian pill, HPLC-DAD, Alkaloids, Flavonoids, Triterpene saponins

CLC Number:

R927.2

Supporting:

Li-Jun Song, Xiao-Mei Tan, Wen-Chang Zhao, Jia-Bo Luo^*. Simultaneous analysis of 12 bioactive constituents in Gegen Qinlian pill by HPLC-DAD [J]. , DOI: 10.5246/jcps.2010.06.062.

[1]	Neha Dangi, Shikha Sharma. Cancer chemotherapy with novel bioactive natural products [J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(8): 589-607.
[2]	Xiangfei Jin, Minyue Cheng, Sihui Zhong, Yungen Xu, Weiyang Shen. Identification of unknown impurities in triamcinolone acetonide palmitate [J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(4): 270-278.
[3]	Liangyu Liu, Yuke Yang, Xiao Du, Tong Wu, Jiannong Wang. Three previously undescribed steroidal glycoalkaloids from Solanum lyratum and their anti-tumor activities [J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(3): 192-201.
[4]	Tong Wu, Xiao Du, Jiannong Wang, Liangyu Liu, Yuke Yang. Two new glycoalkaloids from Solanum lyratum Thunb. [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(6): 518-523.
[5]	Zipeng Qiao, Qizhi Wang, Jianfu Liu, Mingyuan Wang. Antioxidant and antibacterial activities of the ethyl acetate extracts of endophytic fungi isolated from Ageratum conyzoides L. [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(5): 421-433.
[6]	Jiaqian Chen, Chen Jin, Bolun Xu, Huang Zhan, Rongrong Fu, Fengqin Li, Huilian Huang. Identification of major compounds of total flavonoids from Smilax china and evaluation of anti-inflammatory effect on phenol mucilage-induced pelvic inflammation in rats [J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(2): 157-168.
[7]	Yiming Hua, Xiaowen Zhang, Kewu Zeng, Qingying Zhang, Pengfei Tu. Chemical constituents from the aerial parts of Waltheria indica Linn. [J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(7): 468-475.
[8]	Puming Zhang, Chen Zhang, Kewu Zeng, Yong Jiang, Pengfei Tu. Lignans and flavonoids from Artemisia brachyloba [J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(6): 429-435.
[9]	Yifan Zhao, Dong Zhang, Caixia Liang, Lixin Yang, Peng Sun, Yue Ma, Kun Wang, Xiaoqiang Chang, Lan Yang. Chemical constituents from Matricaria chamomilla L. (I) [J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(5): 324-331.
[10]	Lingkun Tong, Hong Wang, Xiaotian Zhang, Ningning Guo, Ya Han, Daidong Wang, Shizhong Chen. Study on chemical constituents of Apocynum venetum L. leaves by LC/MS and determination of the best harvest season [J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(4): 251-262.
[11]	Yongfan Ma, Yanxing Jia. Synthetic study toward the total synthesis of fumigaclavines A–D [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(7): 496-503.
[12]	Feng Pan, Wei Wu, Pinli Dong, Bo Hu, Lingliang Guan, Ling Shu. Simultaneous determination of 10 nucleosides and nucleobases from different cultivation years of Fritillaria unibracteata var. wabuensis by HPLC-DAD [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(5): 346-354.
[13]	Haixiu Jiang, Yanqing Guan, Xiaotian Zhang, Ya Han, Ningning Guo, Hong Wang, Shizhong Chen. Analysis on molecular interaction mechanism of four hapten flavonoids in Shuang-huang-lian powder injection with bovine serum albumin [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(5): 366-371.
[14]	Ling Cheng, Xiaohan Wang, Baode Shen, Pinghua Xu, Chengying Sheng, Juan Zheng, Weibo Liao, Rong Xu, Hailong Yuan. Simultaneous determination of seven bioactive constituents in Liuwei Wuling tablet by HPLC coupled with DAD [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(4): 304-311.
[15]	Gongwen Liang, Na Li, Liping Ma, Libo Zhao, Luwen Shi. HPLC-MS/MS quantification of the HIV-1 protease inhibitor saquinavir in mice plasma and brain [J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(2): 106-114.

Simultaneous analysis of 12 bioactive constituents in Gegen Qinlian pill by HPLC-DAD

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments