Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD

doi:10.5246/jcps.2012.04.046

Abstract

Abstract:

A reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode array detector was established to simultaneously determine nine bioactive compounds in Wuzhuyu decoction, namely dehydroevodiamine, isorhamnetin-7-O-rutinoside, 10-hydroxyrutaecarpine, diosmetin-7-O-β-D-glucopyranoside, evodiamine, rutaecarpine, 1-methyl-2-n-nonyl-4(1H)quinolone, evocarpine, and dihydroevocarpine. The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution. The mobile phases consisted of methanol and water containing 1% (v/v) acetic acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm. All calibration curves showed good linearity (r²>0.9941) within test ranges. The results of intra-day precision, inter-day precision, and accuracy were satisfactory. The overall recoveries were in the range of 90.13%-102.48%. The method was successfully applied to quantify nine bioactive compounds in six samples. Baseline resolution for all nine major compounds was achieved. This method could be used as a suitable quality control assay for Wuzhuyu decoction.

Key words: High-performance liquid chromatography, Wuzhuyu decoction, Alkaloids, Flavonoid glycosides

CLC Number:

R931

Supporting:

Chuan-Qin Hu, Xiu-Wei Yang^* . Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD[J]. , DOI: 10.5246/jcps.2012.04.046.

References

[1] Liu, E.H.; Qi, L.W.; Cheng, X.L.; Peng, Y.B.; Li, P. Biomed. Chromatogr. 2010, 24, 125-131.
[2] Yang, X.W.; Xiao, S.Y.; Yang, Z.; Du, L.J.; Bi, K.S.; Chen, D.W.; Chen, D.F.; Wang, Z.M.; Zou, Y.H. J. Peking Univ (Health Sci.). 2001, 33, 280-282.
[3] Zhao, M.Y.; Yang, X.W. Chromatographia. 2008, 67, 543-550.
[4] Zhao, J.J.; Chen, X.H.; Wang, C.; Wang, Y.J.; Ma, C.; Li, W.; Bi, K.S. Chromatographia. 2010, 72, 187-191.
[5] Ueng, Y.F.; Jan, W.C.; Lin, L.C.; Chen, T.L.; Guengerich, F.P.; Chen, C.F. Drug Metab. Dispos. 2002, 30, 349-353.
[6] Yamahara, J.; Kobayashi, G.; Matsuda, H.; Fujimura, H. Eur. J. Pharmacol. 1988, 155, 139-143.
[7] Ueng, Y.F.; Don, M.J.; Jan, W.C.; Wang, S.Y.; Ho, L.K.; Chen, C.F. Drug Metab. Dispos. 2006, 34, 821-827.
[8] Xie, Y.Y.; Yuan, D.; Yang, J.Y.; Wang, L.H.; Wu, C.F. J. Asian Nat. Prod. Res. 2009, 11, 771-778.
[9] Mohamed, M.; Amira, G.E.; Mona, M.; Mortada, E.S. Z. Naturforsch. C. 2006, 61, 783-791.
[10] Jan, W.C.; Lin, L.C.; Don, M.J.; Chen, C.F.; Tsai, T.H. Biomed. Chromatogr. 2006, 20, 1163-1171.
[11] Zhu, K.F. Lishizhen Med. Mater. Med. Res. 2003, 14, 337-338.
[12] Lee, M.C.; Chuang, W.C.; Sheu, S.J. J. Chromatogr. A. 1996, 755, 113-119.
[13] Chuang, W.C.; Chu, C.Y.; Sheu, S.J. J. Chromatogr. A. 1996, 727, 317-323.
[14] Zhao, Y.; Li, Z.W.; Zhou, X.; Cai, Z.W.; Gong, X.J.; Zhou, C.Y. J. Pharm. Biomed. Anal. 2008, 48, 1230-1236.
[15] Zhao, Y.; Zhou, X.; Chen, H.G.; Gong, X.J.; Cai, Z.W.; Zhou, C.Y. Fitoterapia. 2009, 80, 415-420.
[16] Zhou, Y.; Li, S.H.; Jiang, R.W.; Cai, M.; Liu, X.; Ding, L.S.; Xu, H.X.; Butpaul, P.H.; Shaw, P.C. Rapid Commun. Mass Spectrom. 2006, 20, 3111-3118.
[17] Luo, X.B.; Chen, B.; Yao, S.Z. Talanta. 2005, 66, 103-110.
[18] Xu, H.Y.; Zhang, T.J.; Xiao, X.F.; Zhao, P.; Liu, C.X.; Xu, J. Chin. Herb. Med. 2010, 2, 112-117, 131.
[19] Gong, M.X.; Wang, Y.X.; Song, Y.F.; Wang, Z.M.; Zhang, Q.W.; Wang, W.H.; Zhu, J.J. China J. Chin. Mater. Med. 2010, 35, 1399-1404.
[20] Ning, L.L.; Bi, K.S.; Wang, R.; Che, Z.T.; Wang, X.; Luo, X. Acta Pharm. Sin. 2000, 35, 131-134.

[21] Zhao, D.M.; Zhang, Y.; Bi, K.S. Chin. J. Pharm. Anal. 1999, 19, 16-19.
[22] Gao, X.; Yang, X.W.; Marriott, P.J. J. Sep. Sci. 2010, 33, 2714-2722.
[23] He, D.X.; Shan, Y.; Wu, Y.H.; Liu, G.Z.; Chen, B.; Yao, S.Z. Food Chem. 2011, 127, 880-885.