Preparation and characterization of nanosized ethosomes loaded with tetrandrine and their in vivo efficacy in arthritis treatment

doi:10.5246/jcps.2012.04.042

Abstract

Abstract:

The purpose of this work was to explore the feasibility of ethosomes for improving the anti-arthritic efficacy of topically administered tetrandrine, a bisbenzylisoquinoline alkaloid. Ethosomes were prepared by using the transmembrane pH-gradient loading method and characterized by mean diameter, morphology and entrapment efficiency. The prepared tetrandrine-loaded ethosomes exhibited spherical shape with about 78 nm of average diameter and entrapment efficiency of (52.87±3.81)%, whereas the liposomes had bigger size (99 nm) and higher entrapment efficiency (98.80±0.01)%. In addition, ethosomes exhibited favorable and enhanced penetration behavior as compared with liposomes. More importantly, tetrandrine-loaded ethosomes had a significantly better anti-adjuvant arthritis efficacy in rats compared to liposomes formulation, but no significant difference in the anti-arthritic efficacy between tetrandrine-loaded ethosomes and commercial dexamethasone ointment was observed. These results suggest that ethosomes would be a promising nanocarrier for topical delivery of tetrandrine across skin.

Key words: Tetrandrine, Ethosomes, Liposomes, Anti-arthritic efficacy

CLC Number:

R943

Supporting:

Chao Fan, Xin-Ru Li, Yan-Xia Zhou, Yong Zhao, Wen-Jing Li, Shu-Jin Ma, Yan Liu^*, Gui-Ling Li, Mei Li. Preparation and characterization of nanosized ethosomes loaded with tetrandrine and their in vivo efficacy in arthritis treatment[J]. , DOI: 10.5246/jcps.2012.04.042.

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