Preparation and evaluation of liposomal clarithromycin with antimicrobial effect

doi:10.5246/jcps.2018.06.041

Abstract

Abstract:

In this study, we developed a novel liposomal delivery system modified by Tat peptide and wheat germ agglutinin (WGA) with antimicrobial effect. Physicochemical parameters, in vitro antimicrobial, time-kill study, cellular uptake, biofilm formation inhibition and in vivo antibacterial efficacy against Methicillin-resistant Staphylococcus aureus (MRSA) were investigated. Minimum inhibitory concentrations (MICs) and colony-forming units (CFUs) in the time-kill study for Tat-WGA-modified liposomal clarithromycin (CLA-TatWGALip) were lower than those of free and other modified liposomal CLA. Flow cytometry analysis disclosed that TatWGALip delivered more coumarin 6 into bacteria. Furthermore, Tat-WGA-modified liposomal CLA efficiently inhibited the formation of MRSA biofiom. CFU of MRSA in the abscess of mice treated with CLA-TatWGALip was significantly lower than that of any others (P<0.01). Collectively, liposomal delivery system modified by Tat and WGA could be a promising anti-resistant infection strategy.

Key words: Methicillin-resistant Staphylococcus aureus, Antimicrobial, Liposomes, Clarithromycin, Biofilm

CLC Number:

R944

Supporting:

Wenxi Zhang, Guanghua Peng, Maoyuan Song, Jiaxing Wang, Mengya Yin, Jiajia Li, Yajie Liu, Yuanyuan Zhang, Xinru Li. Preparation and evaluation of liposomal clarithromycin with antimicrobial effect[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(6): 408-414.

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