染料木素和槲皮素体外抗肝纤维化作用

染料木素和槲皮素体外抗肝纤维化作用

齐荔红, 康鲁平, 张俊平^*, 史宁, 张珉, 吴堂明

1.福州总院药剂科,福州350001;
2.第二军医大学药学院生化药学教研室,上海200433;

收稿日期:2001-09-08 修回日期:2001-10-25 出版日期:2001-12-15 发布日期:2001-12-15
通讯作者: 张俊平*

Antifibrotic Effects of Genistein and Quercetin In Vitro

Qi Lihong, Kang Luping, Zhang Junping^*, Shi Ning, Zhang Min, Wu Tangming

1. Department of Pharmacy, Fuzhou General Hospital, Fuzhou 350001;
2. Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433

Received:2001-09-08 Revised:2001-10-25 Online:2001-12-15 Published:2001-12-15
Contact: Zhang Junping*

摘要/Abstract

摘要： 目的研究染料木素和槲皮素对体外肝纤维化的保护作用。方法细胞增殖和胶原合成分别用结晶紫染色法和3H-脯氨酸参入法。原胶原mRNA水平用RT-PCR法测定。结果染料木素(25~70 μmol·L^-1)和槲皮素(6.25~50 μmol·L^-1)浓度依赖抑制PDGF促HSC-T6细胞增殖和TGFβ1刺激的胶原合成及I型原胶原mRNA的表达。结论染料木素和槲皮素抑制PDGF和TGFβ1对星状细胞的作用可能对肝纤维化有保护作用。

关键词: 染料木素, 槲皮素, 抗肝纤维化作用

Abstract: Objective: To study the antifibrotic effects of genistein(GE) and quercetin(QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I procollagen messenger RNA (mRNA) expression stimulated with transforming growth factor β1 (TGFβ1). Methods: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by ³H-proline incorporation assay. Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). Results: GE (25~70 μmol·L^-1) and QU (6.25~50 μmol·L^-1) concentration-dependently attenuated PDGF-driven HSC-T6 cell proliferative activity. TGFβ1-stimulated collagen synthesis was also reduced. This was associated with a decrease in type I procollagen mRNA expression, indicating an effect at a pretranslational level. Conclusion: GE and QU may have therapeutic potential against liver fibrosis by regulating PDGF and TGFβ1 actions.

Key words: Genistein, Genistein, Quercetin, Quercetin, Antifibrotic effects, Antifibrotic effects

Supporting: ^*Correspondence to Zhang Junping. Tel:(021)25070349. E-mail:zjp@smmu.edu.cn
Project supported by the National Natural Science Foundation of China, No: 39670837. First published in Chinese in Acta Pharmaceutical Sinica, 2001, 36:648.

齐荔红, 康鲁平, 张俊平^*, 史宁, 张珉, 吴堂明. 染料木素和槲皮素体外抗肝纤维化作用[J]. .

Qi Lihong, Kang Luping, Zhang Junping^*, Shi Ning, Zhang Min, Wu Tangming . Antifibrotic Effects of Genistein and Quercetin In Vitro[J]. .

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