阿魏酸钠抑制LDL氧化修饰及其致兔主动脉平滑肌细胞的增殖

阿魏酸钠抑制LDL氧化修饰及其致兔主动脉平滑肌细胞的增殖

吴东方, 喻红, 罗顺德

1.武汉大学人民医院药学部, 武汉 430060;
2.武汉大学医学院生物化学教研室, 武汉 430071

收稿日期:2001-05-09 修回日期:2001-07-19 出版日期:2001-12-15 发布日期:2001-12-15

Inhibitory Effects of Sodium Ferulate on Proliferation of Rabbit Aortic Smooth Muscle Cells Induced by Oxidized LDL

Wu Dongfang, Yu Hong, Luo Shunde

1. Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060;
2. Department of Biochemistry, Medical College of Wuhan University, Wuhan 430071

Received:2001-05-09 Revised:2001-07-19 Online:2001-12-15 Published:2001-12-15

摘要/Abstract

摘要： 本文观察阿魏酸钠(SF)对细胞诱导的人低密度脂蛋白(LDL)的氧化修饰及兔主动脉平滑肌细胞(SMC)增殖的作用。在LDL存在的培养的SMC系统中, SF可明显抑制Cu²⁺诱导的MDA含量增高,剂量在40~120 μg·mL^-1内呈剂量依赖性。同时, SF 40~120 μg·mL^-1可降低3H-TdR 细胞掺入量及MTT法显示细胞数降低,提示SF作为抗氧化剂可抑制LDL 的氧化修饰及SMC 的增殖。

关键词: 阿魏酸钠, 氧化/抗氧化, 平滑肌细胞, 动脉粥样硬化

Abstract: The effects of sodium ferulate(SF), a water-soluble element of Chinese medicine Angelica sinensis diels, on cell-mediated oxidative modification of human low density lipoprotein(LDL) and proliferation of rabbit aortic smooth muscle cells(SMCs) were investigated. Using experimental models of proliferation of cultured rabbit aortic SMCs induced by oxidized LDL(ox-LDL), the extent of oxidation was determined by thiobarbituric acid reactive substances(TBARS) method, MTT colorimetry and ³H-thymidine(³H-TdR) incorporation were used to observe proliferation of SMCs. It showed that SF effectively inhibited cell-mediated oxidation induced by Cu²⁺ in a concentration-dependent manner. At the final concentration of 40, 80, 120 μg·mL^-1, SF could significantly inhibit ³H-TdR incorporation and cell Proliferation in a dose-dependent manner. The results indicated that SF could, in vitro protect LDL against oxidative modification and inhibit the proliferation of SMC, which might be due to its free radical scavenging capacity.

Key words: Sodium ferulate, Sodium ferulate, Oxidation, Oxidation, Antioxidant, Antioxidant, Smooth muscle cells, Smooth muscle cells, Atherosclerosis, Atherosclerosis

Supporting:

吴东方, 喻红, 罗顺德. 阿魏酸钠抑制LDL氧化修饰及其致兔主动脉平滑肌细胞的增殖[J]. .

Wu Dongfang, Yu Hong, Luo Shunde . Inhibitory Effects of Sodium Ferulate on Proliferation of Rabbit Aortic Smooth Muscle Cells Induced by Oxidized LDL[J]. .

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