2,4-二硝基苯酚荷移分光光度法测定氯氮卓的含量

2,4-二硝基苯酚荷移分光光度法测定氯氮卓的含量

李彦威, 赵彦生, 宣春生, 魏文珑

太原理工大学化学工程与技术学院, 太原 030024

收稿日期:2001-05-26 修回日期:2001-08-27 出版日期:2001-12-15 发布日期:2001-12-15

Spectrophotometric Determination of Chlordiazepoxide Using Its Charge-transfer Complex with 2,4-Dinitrophenol

Li Yanwei, Zhao Yansheng, Xuan Chunsheng, Wei Wenlong

College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan 030024

Received:2001-05-26 Revised:2001-08-27 Online:2001-12-15 Published:2001-12-15

摘要/Abstract

摘要： 本文报道了基于氯氮卓与2,4-二硝基苯酚在水溶液中的荷移反应测定氯氮卓含量的方法。详细研究了荷移反应的条件和影响荷移反应的因素,结果表明, 氯氮卓与2,4-二硝基苯酚于20°C水溶液中恒温20分钟即可形成1:1的络合物; 在其最大吸收波长444 nm处表观摩尔吸光系数为e = 1.48103 L·mol^-1·cm^-1; 并在2.8~96 mg·mL^-1范围内符合比尔定律; 方法的相对标准偏差为0.32%。将拟定的方法应用于氯氮卓片剂样品含量的测定,并与药典法相比较,结果基本一致。

关键词: 氯氮卓, 2,4-二硝基苯酚, 荷移反应, 分光光度法

Abstract: A new simple, rapid and sensitive spectrophotometric method for the determination of chlordiazepoxide is described, based on the reaction with 2,4-dinitrophenol in water medium, apparently by a charge-transfer mechanism, to yield 1:1 complex with maximum absorption at 444 nm. Optimum experimental conditions for the determination have been studied. The linear calibration range, apparent molar absorptivity and relative standard deviation are 2.8~96.0 mg·mL^-1, 1.48103 and 0.32%, respectively. The method is accurate and has been successfully applied to the determination of chlordiazpoxide in tablets. The results are in good agreement with those obtained with the official method.

Key words: Chlordiazepoxide, Chlordiazepoxide, 2,4-Dinitrophenol, 2,4-Dinitrophenol, Charge-trandfer reaction, Charge-trandfer reaction, Spectrophotometry, Spectrophotometry

Supporting:

李彦威, 赵彦生, 宣春生, 魏文珑. 2,4-二硝基苯酚荷移分光光度法测定氯氮卓的含量[J]. .

Li Yanwei, Zhao Yansheng, Xuan Chunsheng, Wei Wenlong . Spectrophotometric Determination of Chlordiazepoxide Using Its Charge-transfer Complex with 2,4-Dinitrophenol[J]. .

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