刺囊毛霉对天麻素的生物转化研究

刺囊毛霉对天麻素的生物转化研究

占纪勋, 郭洪祝, 戴均贵, 张元兴, 果德安^*

1.北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100083;
2.华东理工大学生物反应器工程国家重点实验室, 上海 200237

收稿日期:2001-05-08 修回日期:2001-09-05 出版日期:2001-12-15 发布日期:2001-12-15
通讯作者: 果德安*

Biotransformation of Gastrodin by Mucor spinosus

Zhan Jixun, Guo Hongzhu, Dai Jungui, Zhang Yuanxing, Guo Dean^*

1. The State Key Laboratory of Natural and Biominetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083;
2. The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237

Received:2001-05-08 Revised:2001-09-05 Online:2001-12-15 Published:2001-12-15
Contact: Guo Dean*

摘要/Abstract

摘要： 利用刺囊毛霉(Mucor spinosus)3.3450的培养液对天麻素进行生物转化,得到其酶水解产物甙元对羟基苯甲醇,转化率接近100%。通过无细胞抽提液对底物转化的研究,证明催化该反应的酶为组成酶、胞外酶。

关键词: 刺囊毛霉, 生物转化, 天麻素, 对羟基苯甲醇

Abstract: Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 percent. This product was identified as p-hydroxy benzyl alcohol on the basis of its ¹H, ¹³C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransforma-tion reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.

Key words: Mucor spinosus, Mucor spinosus, Biotransformation, Biotransformation, Gastrodin, Gastrodin, p-Hydroxy benzyl alcohol, p-Hydroxy benzyl alcohol

Supporting:

占纪勋, 郭洪祝, 戴均贵, 张元兴, 果德安^*. 刺囊毛霉对天麻素的生物转化研究[J]. .

Zhan Jixun, Guo Hongzhu, Dai Jungui, Zhang Yuanxing, Guo Dean^*. Biotransformation of Gastrodin by Mucor spinosus[J]. .

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