中药成分PE对老年人淋巴细胞凋亡的调控作用

中药成分PE对老年人淋巴细胞凋亡的调控作用

李玉梅, 张春玲, 范芸, 刘俊达^*

1.广州老人院, 广州 510550;
2.卫生部北京医院老年医学研究所, 100730

收稿日期:2003-03-05 修回日期:2003-08-10 出版日期:2003-09-15 发布日期:2003-09-15
通讯作者: 刘俊达*

Modulatory Effect of Pollen Extract on Apoptosis of Lymphocytes in the Elderly

LI Yu-mei , ZHANG Chun-ling, FAN Yun, LIU Jun-da^*

1.Guangzhou aged Home, Guangzhou 510550, China;
2.Beijing Hospital, Beijing 100730, China

Received:2003-03-05 Revised:2003-08-10 Online:2003-09-15 Published:2003-09-15
Contact: LIU Jun-da*

摘要/Abstract

摘要： 目的研究老年人淋巴细胞亚群及其细胞凋亡过程, 探讨中药成分PE对细胞凋亡的调控作用.方法用间接免疫荧光技术检测淋巴细胞表型, 用MTT法测定淋巴细胞增殖应答; 用流式细胞仪及自动图象分析来检测细胞凋亡.结果老年人淋巴细胞比青年人淋巴细胞的增殖应答能力低, CD45 RA+细胞亚群减少, 而CD45 RA+细胞亚群增多, 后者易发生凋亡.而中药成分PE对老年人淋巴细胞凋亡有抑制作用.结论老年人淋巴细胞易于细胞凋亡, 取决于活化, 称为活化诱导性细胞死亡, 此在免疫衰老中起着重要作用.研究结果启示可从中药开发细胞凋亡的调节药物.

关键词: 中药成分PE, 淋巴细胞, 细胞凋亡, CD45 RA+细胞, 自动图象分析

Abstract: Aim In this study, we investigated the changes of lymphocyte subpopulation and apoptosis process of lymphocytes in the elderly, and the modulatory effect of pollen extract (PE) on apoptosis. Methods Lymphocyte phenotypes were detected using indirect immunofluorescence technique. The proliferation responses were determined by MTT method. Flow cytometry and automatic image analysis were performed to evaluate the apoptosis of lymphocytes. Results The proliferation responses of lymphocytes in the elderly were lower than that in young adults. Decreased CD45 RA+ cells and increased CD45 RA+ cells were found in lymphocyte population of aged people, compared with that of young adults. The CD45 RA+ cells were prone to apoptosis. There is an inhibitory effect of PE on apoptosis of lymphocytes in the elderly. Conclusion It is implied that the susceptibility of lymphocyte in the elderly to apoptosis depends on activation, so called, activation-induced cell death. Present results suggest that apoptosis of lymphocytes in aged people play an important role in the pathogenesis of immunosenescence. Thus, a possibility is open for development of apoptosis-modulating drugs from pollen.

Key words: pollen extract, pollen extract, lymphocytes, lymphocytes, apoptosis, apoptosis, CD45 RA+ cells, CD45 RA+ cells, automatic image analysis, automatic image analysis

中图分类号:

R285.5

R967

Supporting: Foundation item: Supporded by the National Natural Science of China, No.39770860.
^*Corresponding author. Tel.: 13693261322

李玉梅, 张春玲, 范芸, 刘俊达^*. 中药成分PE对老年人淋巴细胞凋亡的调控作用[J]. .

LI Yu-mei , ZHANG Chun-ling, FAN Yun, LIU Jun-da^* . Modulatory Effect of Pollen Extract on Apoptosis of Lymphocytes in the Elderly[J]. .

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