补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究

补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究

贺文彬, 张俊龙, 陈乃宏, 张岭, 朱海波^*

1.山西中医学院, 山西太原 030024;
2.中国医学科学院中国协和医科大学药物研究所, 北京 100050

收稿日期:2004-07-20 修回日期:2005-05-10 出版日期:2005-06-15 发布日期:2005-06-15
通讯作者: 朱海波*

Neuroprotective Effects of Bushen Decoction Against Glutamate-Induced Neurotoxicity in PC12 Cells

HE Wen-bin, ZHANG Jun-long, CHEN Nai-hong, ZHANG ling, ZHU Hai-bo^*

1.Shanxi College of Traditional Chinese Medicine, Taiyuan 030024, China;
2.Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

Received:2004-07-20 Revised:2005-05-10 Online:2005-06-15 Published:2005-06-15
Contact: ZHU Hai-bo*

摘要/Abstract

摘要： 目的运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用。方法以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用, 采用流式细胞仪观察BS对谷氨酸所诱导PC12细胞凋亡的影响。结果 BS含药血清可以增强PC12细胞活力, 拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强, 并且能够抑制谷氨酸诱导的PC12细胞早期凋亡。结论补肾方BS可能具有神经保护作用。

关键词: 补肾复方, 补肾复方, 补肾复方, 神经保护, 神经保护, 神经保护, 细胞凋亡, 细胞凋亡, 细胞凋亡, 中药血清药理学, 中药血清药理学, 中药血清药理学, PC12细胞, PC12细胞, PC12细胞

Abstract: Aim The enhanced effect of Bushen (Kidney-tonifying) decoction (BS) on cultured PC12 cell proliferation and its antagonistic action on neurotoxicity induced by glutamate were investigated by serum pharmacological method of the Chinese material medica (CMM) in vitro. Methods The effect of BS on cultured PC12 cell activity and its antagonistic action on neurotoxicity induced by glutamate was observed by MTT method. Flow cytometry and fluorescence microscope techniques were employed to observe the antagonistic effect of BS on early period apoptosis of PC12 cells induced by glutamate. Results The serum with BS was able to enhance activity of PC12 cells and exert antagonistic effect on glutamate-induced neurotoxicity. Meanwhile, these beneficial effects produced by BS were found to be the strongest in 20% concentration of in serum BS. Moreover, it can inhibit apoptosis of PC12 cells induced by glutamate, which occurs in the early period. Conclusion BS may exert a potential neuroprotective effect.

Key words: Bushen decoction, Bushen decoction, neuroprotective effect, neuroprotective effect, apoptosis, apoptosis, pharmacology Chinese material medica, pharmacology Chinese material medica, PC12 cells, PC12 cells

中图分类号:

R285

R285.5

Supporting: Foundation item: National Natural Science Foundation of Shanxi Province (No:19991091) and Hi-Tech Resereh and Development Program of China (No: 2004AA2Z3815).
^*Corresponding author. Tel.: 86-10-63188106; fax: 86-10-63017757

贺文彬, 张俊龙, 陈乃宏, 张岭, 朱海波^*. 补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究[J]. .

HE Wen-bin, ZHANG Jun-long, CHEN Nai-hong, ZHANG ling, ZHU Hai-bo^*. Neuroprotective Effects of Bushen Decoction Against Glutamate-Induced Neurotoxicity in PC12 Cells[J]. .

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