一标多测法同时测定肉苁蓉中5种苯乙醇苷类成分的含量

doi:10.5246/jcps.2019.08.051

中国药学(英文版) ›› 2019, Vol. 28 ›› Issue (8): 537-546.DOI: 10.5246/jcps.2019.08.051

一标多测法同时测定肉苁蓉中5种苯乙醇苷类成分的含量

李瑞燕¹, 赵明波², 屠鹏飞², 姜勇^2*

1. 长治医学院药学系, 山西长治 046000
2. 北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100191

收稿日期:2019-05-18 修回日期:2019-06-02 出版日期:2019-09-02 发布日期:2019-06-15
通讯作者: Tel.: +86-10-82802719, E-mail: yongjiang@bjmu.edu.cn
基金资助:
National Key R & D Program of China (Grant Nos. 2017YFC1702400, 2018YFC1707300 and 2018YFC1707904).

Simultaneous determination of five phenylethanoid glycosides in Cistanches Herba using quantitative analysis of multi-components by single marker

Ruiyan Li¹, Mingbo Zhao², Pengfei Tu², Yong Jiang^2*

1. Department of Pharmacy, Changzhi Medical College, Changzhi 046000, China
2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China

Received:2019-05-18 Revised:2019-06-02 Online:2019-09-02 Published:2019-06-15
Contact: Tel.: +86-10-82802719, E-mail: yongjiang@bjmu.edu.cn
Supported by:
National Key R & D Program of China (Grant Nos. 2017YFC1702400, 2018YFC1707300 and 2018YFC1707904).

摘要/Abstract

摘要：

本研究建立了同时测定肉苁蓉中松果菊苷、管花苷A、类叶升麻苷、异类叶升麻苷和2'-乙酰毛蕊花糖苷5种苯乙醇苷类成分含量的一标多测方法(quantitative analysis of multi-components by single marker, QAMS)。以松果菊苷为内标, 计算其他4种成分相对于该成分的相对校正因子(relative correction factor, RCFs), 并对影响RCFs的各种因素进行了考察; 然后利用所得RCFs计算各成分的含量, 并与外标法测定结果进行比较, 以验证一测多评法的可行性和准确性。通过对10批肉苁蓉药材中5种苯乙醇苷类成分的测定, 分别用校正因子法与外标法计算含量, 结果发现2种方法测定的结果无显著性差异, 证明了采用QAMS测定中药肉苁蓉中苯乙醇苷类成分的含量准确、可行, 为肉苁蓉药材的质量控制提供了经济有效的方法。

关键词: 肉苁蓉, 苯乙醇苷类, 一标多测, 相对校正因子

Abstract:

In the present study, a method for the quantitative analysis of multi-components by single marker (QAMS)has been developed and validated for the simultaneous determination of echinacoside (ECH), tubuloside A, acteoside, isoacteoside, and 2'-acetylacteoside inCistanches Herba. ECH was used as the internal standard (IS) to obtain the relative correction factors (RCFs) of the other four phenylethanoid glycosides (PhGs); meanwhile, various influencing factors on RCFs were investigated under differentconditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method (ESM) to verify the feasibility and accuracy of the established QAMS method. No significant differencewas found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM.The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.

Key words: Cistanches Herba, Phenylethanoid glycosides, Quantitative analysis of multi-components by single-marker, Relative correction factors

中图分类号:

R917

Supporting:

李瑞燕, 赵明波, 屠鹏飞, 姜勇. 一标多测法同时测定肉苁蓉中5种苯乙醇苷类成分的含量[J]. 中国药学(英文版), 2019, 28(8): 537-546.

Ruiyan Li, Mingbo Zhao, Pengfei Tu, Yong Jiang. Simultaneous determination of five phenylethanoid glycosides in Cistanches Herba using quantitative analysis of multi-components by single marker[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(8): 537-546.

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一标多测法同时测定肉苁蓉中5种苯乙醇苷类成分的含量

Simultaneous determination of five phenylethanoid glycosides in Cistanches Herba using quantitative analysis of multi-components by single marker

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