绿原酸、新绿原酸、隐绿原酸与溶菌酶相互作用的荧光光谱法研究

doi:10.5246/jcps.2014.08.070

中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (8): 543-547.DOI: 10.5246/jcps.2014.08.070

绿原酸、新绿原酸、隐绿原酸与溶菌酶相互作用的荧光光谱法研究

兰月香, 刘梅仙, 陈世忠, 王弘^*

北京大学医学部药学院天然药物化学系, 北京 100191

收稿日期:2014-05-07 修回日期:2014-05-14 出版日期:2014-08-31 发布日期:2014-05-27
通讯作者: Tel./Fax: 86-10-82801559
基金资助:
Beijing Natural Science Foundation (Grant No. 7142088).

Spectrophotometric studies on the interaction between chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and lysozyme

Yuexiang Lan, Meixian Liu, Shizhong Chen, Hong Wang^*

Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2014-05-07 Revised:2014-05-14 Online:2014-08-31 Published:2014-05-27
Contact: Tel./Fax: 86-10-82801559
Supported by:
Beijing Natural Science Foundation (Grant No. 7142088).

摘要/Abstract

摘要：

用荧光光谱法研究了绿原酸、新绿原酸、隐绿原酸与溶菌酶之间的相互作用。绿原酸(CA)、新绿原酸(NCA)、隐绿原酸(CCA)均能显著焠灭溶菌酶的内源荧光并以静态焠灭为主; 随着温度的升高其结合常数和结合位点均呈现降低的趋势。根据热力学参数判断确定CA与LYSO之间以疏水作用力为主, NCA与LYSO之间以氢键和范德华力为主, CCA与LYSO之间以静电作用力为主。

关键词: 绿原酸, 新绿原酸, 隐绿原酸, 溶菌酶, 荧光光谱法

Abstract:

The interactions of chlorogenic acid (CA), neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA) with lysozyme (LYSO) were investigated in physiological buffer by fluorescence spectroscopy. The mechanism study indicated that CA, NCA and CCA could strongly quench the intrinsic fluorescence of LYSO through static quenching procedures withone binding site. Thermodynamic data show that the major force in the binding processes of CA to LYSO was hydrophobicinteractions; for NCA, it was the hydrogen bonds and van der Waals forces, as for the CCA system, the mainly force is electrostatic force.

Key words: Chlorogenic acid, Neochlorogenic acid, Cryptochlorogenic acid, Lysozyme, Fluorescence quenching

中图分类号:

R917

Supporting:

兰月香, 刘梅仙, 陈世忠, 王弘. 绿原酸、新绿原酸、隐绿原酸与溶菌酶相互作用的荧光光谱法研究[J]. 中国药学(英文版), 2014, 23(8): 543-547.

Yuexiang Lan, Meixian Liu, Shizhong Chen, Hong Wang. Spectrophotometric studies on the interaction between chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and lysozyme[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(8): 543-547.

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绿原酸、新绿原酸、隐绿原酸与溶菌酶相互作用的荧光光谱法研究

Spectrophotometric studies on the interaction between chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and lysozyme

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