HPLC-DAD及HPLC-ESI-MS/MS法对马齿苋环多巴酰胺的定性定量研究

doi:10.5246/jcps.2014.08.069

中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (8): 533-542.DOI: 10.5246/jcps.2014.08.069

HPLC-DAD及HPLC-ESI-MS/MS法对马齿苋环多巴酰胺的定性定量研究

焦泽沼, 王海钠, 王培培, 孙洪祥, 岳苏, 向兰^*

山东大学药学院, 山东济南 250012

收稿日期:2014-04-08 修回日期:2014-04-21 出版日期:2014-08-31 发布日期:2014-05-10
通讯作者: Tel.: 86-531-88382028, Fax: 86-531-88382548
基金资助:
National Natural Science Foundation of China (Grant No. 81073005), Shandong Province Young and Middle-Aged Scientists Research Awards Fund (Grant No. BS2010YY032), Scientific Research Foundation for Returned Overseas Scholars, Ministry of Education of China (Grant No. 42) and Innovation Project of Shandong University (Grant No. 2012TS102).

Detection and quantification of cyclo-dopa amides in Portulaca oleracea L. by HPLC-DAD and HPLC-ESI-MS/MS

Zezhao Jiao, Haina Wang, Peipei Wang, Hongxiang Sun, Su Yue, Lan Xiang^*

School of Pharmaceutical Sciences, Shandong University, Shandong Jinan 250012, China

Received:2014-04-08 Revised:2014-04-21 Online:2014-08-31 Published:2014-05-10
Contact: Tel.: 86-531-88382028, Fax: 86-531-88382548
Supported by:
National Natural Science Foundation of China (Grant No. 81073005), Shandong Province Young and Middle-Aged Scientists Research Awards Fund (Grant No. BS2010YY032), Scientific Research Foundation for Returned Overseas Scholars, Ministry of Education of China (Grant No. 42) and Innovation Project of Shandong University (Grant No. 2012TS102).

摘要/Abstract

摘要：

对中药马齿苋中环多巴酰胺类生物碱进行定性定量分析。采用HPLC-DAD及改进的HPLC-ESI-MS/MS方法, 从马齿苋中鉴定了8个新环多巴酰胺Oleracein H-O和4个已知环多巴酰胺Oleracein A-D。通过分析11批样品, 首次建立了马齿苋药材环多巴酰胺的HPLC指纹图谱, 并同时对2个主成分Oleracein A (OA)和Oleracein B (OB)进行了定量研究, 对总环多巴酰胺进行了半定量分析, 结果表明, 十一批马齿苋药材中OA和OB的含量分别为35.00-151.93 mg/kg和 40.00-150.44 mg/kg, 总环多巴酰胺的含量分别为314.16-928.60 mg/kg (以OA计算) 或475.83-1393.00 mg/kg (以OB计算)。本实验结果为马齿苋药材的质量评价和质量控制提供了新方法。

关键词: 马齿苋, 环多巴酰胺, 定性定量研究

Abstract:

In the present study, we aimed to detect and quantify cyclo-dopa amides in Portulaca oleracea L. Together with four known ones (oleracein A–D), we further identified eight new cyclo-dopa amides, named oleracein H–O, using an HPLC-DAD combined with an improved HPLC-ESI-MS/MS method. A standardized HPLC fingerprint of cyclo-dopa amides was generated for the first time through analyzing 11 batches of P. oleracea. Simultaneously, the contents of two major amides, oleracein A (OA) and oleracein B (OB), and the total amides (TID) were determined. Our results showed that the contents of OA and OB of P. oleracea ranged from 35.00 to 151.93 mg/kg and from 40.00 to 150.44 mg/kg, respectively. Moreover, TID ranged from 314.16 to 928.60 mg/kg (calculated by OA), or from 475.83 to 1393.00 mg/kg (calculated by OB). Taken together, our newly developed method could be used for the quality assessment and quality control of this herb.

Key words: Portulaca oleracea L., Cyclo-dopa amide, Detection and quantification

中图分类号:

R917

Supporting:

焦泽沼, 王海钠, 王培培, 孙洪祥, 岳苏, 向兰. HPLC-DAD及HPLC-ESI-MS/MS法对马齿苋环多巴酰胺的定性定量研究[J]. 中国药学(英文版), 2014, 23(8): 533-542.

Zezhao Jiao, Haina Wang, Peipei Wang, Hongxiang Sun, Su Yue, Lan Xiang. Detection and quantification of cyclo-dopa amides in Portulaca oleracea L. by HPLC-DAD and HPLC-ESI-MS/MS[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(8): 533-542.

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HPLC-DAD及HPLC-ESI-MS/MS法对马齿苋环多巴酰胺的定性定量研究

Detection and quantification of cyclo-dopa amides in Portulaca oleracea L. by HPLC-DAD and HPLC-ESI-MS/MS

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