多成分、多途径同时干预提供强效抗脑缺血作用

doi:10.5246/jcps.2014.05.040

中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (5): 295-301.DOI: 10.5246/jcps.2014.05.040

多成分、多途径同时干预提供强效抗脑缺血作用

张精亮, 胡涛, 刘晓岩, 朱元军, 王银叶^*

北京大学医学部药学院分子与细胞药理学系, 北京 100191

收稿日期:2012-02-25 修回日期:2014-03-11 出版日期:2014-05-23 发布日期:2014-03-24
通讯作者: Tel.: 86-10-82802652; E-mail: wangyinye@bjmu.edu.cn
基金资助:
National Natural Science Foundation of China (Grant No. 81302763) and Beijing Natural Science Foundation (Grant No. 7144218).

Intervention of multiple pathways by multiple active components provides potent protection against cerebral ischemia injury

Jingliang Zhang, Tao Hu, Xiaoyan Liu, Yuanjun Zhu, Yinye Wang^*

Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2012-02-25 Revised:2014-03-11 Online:2014-05-23 Published:2014-03-24
Contact: Tel.: 86-10-82802652; E-mail: wangyinye@bjmu.edu.cn
Supported by:
National Natural Science Foundation of China (Grant No. 81302763) and Beijing Natural Science Foundation (Grant No. 7144218).

摘要/Abstract

摘要：

为探索多成分、多途径干预急性脑缺血损伤的效果, 用筛选出的中药复方-复方2为工具药(主要成分: 人参皂苷, 葛根黄酮, 麦冬皂苷) 进行研究。首先研究复方本身及其各组分对损伤的PC12细胞存活率和潜在机制, 观察复方2对血栓形成和血小板聚集的影响, 最后研究复方2对大鼠脑缺血再灌注损伤的影响。实验结果表明, 复方2明显增强H₂O₂诱导的PC12细胞存活率, 活性强于各组分; 显著减少H₂O₂诱导的PC12细胞NO的产生, 作用强于各组分; 能增强SOD活性, 其活性强于人参皂苷; 降低MDA含量, 其活性强于麦冬皂苷。体内实验表明, 复方2能有效地抑制血小板聚集和静脉血栓形成。复方2皮下单剂量给药可明显减小脑缺血再灌注大鼠的脑梗死体积, 并降低神经功能评分, 表明复方2具有很强的抗脑缺血作用, 这种作用可能是由多种成分通过多种途径同时干预而产生的。

关键词: 多种途径, 多种成分, 脑缺血损伤, 复方2

Abstract:

To explore the effect of multiple pathway intervention in acute cerebral ischemia injury, we prepared a medicine formula (formula 2) consisting of ginsenosides, pueraria flavonoids, ophiopogonis and borneol as a tool medicine. The effects of formula 2 and its components on PC12 cell viability and potential pathway were investigated, and the influence of this formula on venous thrombosis and platelet aggregation was also assessed, then the effect of formula 2 on middle cerebral artery occlusion (MCAO) reperfusion was observed in rats. Formula 2 markedly enhanced the cell viability, which was stronger than that of each individual component. Formula 2 significantly inhibited the NO production in PC12 cells induced by H₂O₂, and this effect was also stronger than that of each individual component. Moreover, formula 2 enhanced the SOD activity, and the effect was stronger than that of ginsenosides. In addition, formula 2 reduced the MDA content, and this effect was stronger than that of ophiopogonins. In vivo, formula 2 showed potent inhibitory effects on platelet aggregation and venous thrombosis. Furthermore, formula 2 (single dose, s.c.) significantly reduced the infarct volume and neurobehavioral scores in MCAO reperfusion rats. Take together, our results suggests that formula 2 has powerful ability of inhibiting the ischemia/reperfusion injury, and this effect might be attributed to its simultaneous intervention in the cascade reaction of neuronal injury via multiple pathways contributed by multiple components during cerebral ischemia/reperfusion.

Key words: Multiple pathways, Multiple components, Cerebral ischemia/reperfusion injury, Formula 2

中图分类号:

R962

Supporting: National Natural Science Foundation of China (Grant No. 81302763) and Beijing Natural Science Foundation (Grant No. 7144218).

张精亮, 胡涛, 刘晓岩, 朱元军, 王银叶. 多成分、多途径同时干预提供强效抗脑缺血作用[J]. 中国药学(英文版), 2014, 23(5): 295-301.

Jingliang Zhang, Tao Hu, Xiaoyan Liu, Yuanjun Zhu, Yinye Wang. Intervention of multiple pathways by multiple active components provides potent protection against cerebral ischemia injury[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(5): 295-301.

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多成分、多途径同时干预提供强效抗脑缺血作用

Intervention of multiple pathways by multiple active components provides potent protection against cerebral ischemia injury

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