超声波辅助溶剂萃取西藏和河南藏红花中挥发油的GC-MS分析

doi:10.5246/jcps.2011.04.051

超声波辅助溶剂萃取西藏和河南藏红花中挥发油的GC-MS分析

贾凌晗, 刘一^*, 李玉珍

1. 中国药科大学药学院, 江苏南京 211198
2. 北京大学人民医院药剂科, 北京 100044

收稿日期:2011-02-18 修回日期:2011-05-20 出版日期:2011-07-01 发布日期:2011-07-01
通讯作者: 刘一*

Analysis of volatile components in saffron from Tibet and Henan by ultrasonic-assisted solvent extraction and GC-MS

Ling-Han Jia, Yi Liu^*, Yu-Zhen Li

1. School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China
2. Department of Pharmacy, Peking University People’s Hospital, Beijing 100044, China

Received:2011-02-18 Revised:2011-05-20 Online:2011-07-01 Published:2011-07-01
Contact: Yi Liu*

摘要/Abstract

摘要：

对西藏和河南藏红花中挥发油化学成分进行提取和分析, 比较不同溶剂萃取藏红花挥发油化学成分的差异。采用超声波辅助溶剂萃取法提取挥发油, 分别采用乙醚、乙醇、乙酸乙酯、二氯甲烷和丙酮5种溶剂萃取后浓缩, 通过GC-MS分析, 实现对组分的鉴定, 同时用峰面积归一化法计算它们的相对含量。结果表明, 不同溶剂提取藏红花中挥发油化学成分间存在差异, 且西藏和河南藏红花中提取所得挥发性成分的组成和比例也存在差异。超声波辅助溶剂萃取/GC-MS是一种简单、快速、有效的挥发油成分分析方法。

关键词: 藏红花, 挥发油, 超声波辅助溶剂萃取, 气相色谱-质谱

Abstract:

To determine the chemical constituents of volatile components in saffron from the Tibet Autonomous Region and the Henan Province of China and to compare the chemical composition difference in the saffron, the total volatile components were extracted by ultrasonic-assisted solvent extraction (USE), using five different solvents: diethyl ether, ethanol, ethyl acetate, dichloromethane and acetone, analyzed using gas chromatography-mass spectrometry (GC-MS) and compared to the standard MS data, and their relative contents were calculated by area normalization. The results showed both that USE was an efficient and rapid method for the extraction of the volatile components from saffron and that the components extracted from the same sample using different solvents were different. Comparison of the experimental results of saffron from the Tibet Autonomous Region and the Henan Province of China showed that their volatile components were different in their chemical composition and in their relative percentages. USE/GC-MS is a simple, rapid, and effective method for the analysis of volatile oil components in saffron.

Key words: Saffron, Volatile components, Ultrasonic-assisted solvent extraction, GC-MS

中图分类号:

R917

Supporting:

^*Corresponding author. Tel.: 86-10-88325021; E-mail: liuyipku@126.com

贾凌晗, 刘一^*, 李玉珍

. 超声波辅助溶剂萃取西藏和河南藏红花中挥发油的GC-MS分析[J]. , DOI: 10.5246/jcps.2011.04.051.

Ling-Han Jia, Yi Liu^*, Yu-Zhen Li. Analysis of volatile components in saffron from Tibet and Henan by ultrasonic-assisted solvent extraction and GC-MS[J]. , DOI: 10.5246/jcps.2011.04.051.

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