覆盆子体外抗炎活性评价

覆盆子体外抗炎活性评价

肖飞, 朱江, 赵雷, 郑国华, 杨安树, 陶君彦^*, 张宝徽, 黄志军 , 熊富良

1. 武汉健民集团, 湖北武汉 430054
2. 江西省食品工业研究所, 江西南昌 330029
3. 华中科技大学协和医学院传染科, 湖北武汉 430022
4. 湖北中医学院药学院, 湖北武汉 430065
5. 南昌大学食品科学与技术国家重点实验室, 江西南昌 330047

收稿日期:2009-09-18 修回日期:2010-03-15 出版日期:2010-05-15 发布日期:2010-05-15
通讯作者: 陶君彦*

Involvement of pro-inflammatory and anti-inflammatory cytokines in the anti-inflammatory activity of Rubus idaeus L. on LPS-treated RAW 264.7 cells

Fei Xiao, Jiang Zhu, Lei Zhao, Guo-Hua Zheng, An-Shu Yang, Jun-Yan Tao^*, Bao-Hui Zhang, Zhi-Jun Huang, Fu-Liang Xiong

1. Hubei & Wuhan Engineering Technology Center of Modernization Traditional Chinese Medicine, Wuhan Jianmin Pharmacy of TCM Co., Ltd, Wuhan 430054, China
2. Jiangxi Institute of Food Industry, Nanchang 330029, China
3. Department of Hepatology & Infectious Disease, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China
4. School of Pharmacy, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China
5. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2009-09-18 Revised:2010-03-15 Online:2010-05-15 Published:2010-05-15
Contact: Jun-Yan Tao*

摘要/Abstract

摘要： 本文采用脂多糖处理的RAW 264.7细胞为炎症细胞模型, 采用ELISA、实时荧光定量PCR、免疫印迹、免疫细胞化学等方法探讨了覆盆子提取物对炎症发生过程中促炎、抗炎细胞因子及NF-κB的影响。结果表明, 覆盆子提取物能够明显抑制脂多糖处理的RAW 264.7细胞中IL-1β、IL-6、NO、TNF-α和COX-2的表达(P<0.01或P<0.05), 增强IL-10、HO-1等抗炎细胞因子的表达 (P<0.01或P<0.05), 并且提取物能够抑制NF-κB的产生。因此, 我们推断覆盆子提取物具有较好的抗炎作用, 其起效的可能机制是通过抑制NF-κB激活来抑制促炎因子的产生, 并促进抗炎因子的表达。

关键词: 覆盆子, RAW 264.7 细胞, NF-κB, 促炎细胞因子, 抗炎细胞因子

Abstract: Anti-inflammatory activity of Rubus idaeus L. and possible mechanisms involved were explored in lipopolysaccharide (LPS)-treated RAW 264.7 cells. The effects of ethanol extract of R. idaeus on the levels of pro-inflammatory cytokines, including tumor necrosis factor-alfa (TNF-α), interleukin-6 (IL-6) and interleukin-1 beta (IL-1β), as well as pro-inflammatory mediators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were studied by Sandwich ELISA, real-time PCR, and Western blot analysis. Moreover, the effects of ethanol extract of R. idaeus on anti-inflammatory cytokine interleukin-10 (IL-10) and anti-inflammatory mediator heme oxygenase-1 (HO-1) were also investigated using the same methods. Furthermore, nuclear factor-κB (NF-κB) level was assayed by immunocytochemistry. The results showed that the production of IL-1β, IL-6, NO, TNF-α and COX-2 in LPS-treated cells could be significantly inhibited (P<0.01 or P<0.05) by ethanol extract of R. idaeus compared with that in the cells treated with LPS only. Meanwhile, the production of NF-κB was also inhibited by the extract. Based on these results, the anti-inflammatory activity of R. idaeus was attributed to the down-regulation of IL-6, IL-1β and TNF-α levels as well as gene expression of iNOS and COX-2 through the suppression of NF-κB activation, and induction of anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1.

Key words: Rubus idaeus L., RAW 264.7 cell, NF-κB, Pro-inflammatory cytokines, Anti-inflammatory cytokines

中图分类号:

R285.5

Supporting: ^*Corresponding author. Tel.: 86-27-68889013

肖飞, 朱江, 赵雷, 郑国华, 杨安树, 陶君彦^*, 张宝徽, 黄志军 , 熊富良. 覆盆子体外抗炎活性评价 [J]. .

Fei Xiao, Jiang Zhu, Lei Zhao, Guo-Hua Zheng, An-Shu Yang, Jun-Yan Tao^*, Bao-Hui Zhang, Zhi-Jun Huang, Fu-Liang Xiong. Involvement of pro-inflammatory and anti-inflammatory cytokines in the anti-inflammatory activity of Rubus idaeus L. on LPS-treated RAW 264.7 cells [J]. .

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