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反相高效液相色谱法测定大鼠尿、粪便和组织中岩白菜素的含量

史彦斌*, 师彦平, 张晓云, 赵全义, 倪京满
  

  1. 1. 兰州大学 药学院, 甘肃 兰州 730000
    2. 中国科学院 兰州化学物理研究所, 甘肃 兰州 730000
  • 收稿日期:2008-10-09 修回日期:2009-02-10 出版日期:2009-03-15 发布日期:2009-03-15
  • 通讯作者: 史彦斌*

Determination of bergenin in rat urine, feces and tissues by RP-HPLC method

Yan-Bin Shi*, Yan-Ping Shi, Xiao-Yun Zhang, Quan-Yi Zhao, Jing-Man Ni
  

  1. 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China
    2. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2008-10-09 Revised:2009-02-10 Online:2009-03-15 Published:2009-03-15
  • Contact: Yan-Bin Shi*

摘要: 本文建立了应用反相高效液相色谱测定大鼠尿、粪便和组织中水溶性药物岩白菜素含量的方法。色谱条件为DiamonsilTM C18谱柱, 甲醇-水为流动相, 流速0.8 mL/min, 柱温40 ºC, 检测波长 220 nm。尿中药物浓度在0.5-100 µg/mL范围内与峰面积呈良好线性关系(r = 0.9989), 绝对回收率介于90.0%-92.6%。肺中药物浓度0.5-150 µg/g范围内, 其它被测组织中药物浓度0.3-150 µg/g 范围内与峰面积具有良好线性关系(r0.9996), 各组织中药物绝对回收率均大于63.9%。该方法用于测定大鼠静脉注射岩白菜素后尿液、粪便和组织中药物的浓度, 结果表明约有35.36%的服药剂量从其尿液中回收, 不足10%的剂量从粪便中回收。原形药物能快速分布到被测组织中, 肾中药物分布最多, 而脑组织中分布最少, 这与药物本身具有较好的水溶性相关。

关键词: 反相高效液相色谱;, 反相高效液相色谱;, 反相高效液相色谱;, 岩白菜素;, 岩白菜素;, 岩白菜素;, 排泄;, 排泄;, 排泄;, 分布, 分布, 分布

Abstract:

A RP-HPLC analytical method was developed for determining bergenin in rat urine, feces and tissues. The separation was performed on a C18 analytical column with methanol-water as the mobile phase at a flow rate of 0.8 mL/min. The column temperature was 40 ºC and the eluate was monitored at 220 nm. The calibration curve for urine analysis showed a good linearity (r = 0.9989) over the range of 0.5-100 µg/mL, and the absolute recoveries were in the range of 90.0%-92.6%. A good linearity (r0.9996) for all tested tissues was obtained over the range of 0.3-150 µg/g except for lung, which had a linearity range of 0.5-150 µg/g. The absolute recoveries from tissues were higher than 63.9%. The method was successfully applied to determine the bergenin levels in several biological samples after rats were given bergenin formulation through tail vein injection. The results demonstrated that the amount of bergenin recovered from urine was about 35.36% of the total dose, and that from feces was less than 10%. Bergenin can be quickly and widely distributed in the rat, accumulating high levels in the kidney in comparison to all other tissues investigated. It was not distributed into the brain at any significant level owing to its hydrophilic property.

Key words: RP-HPLC, RP-HPLC, Bergenin, Bergenin, Excretion, Excretion, Distribution, Distribution

中图分类号: 

Supporting: Foundation items: Natural Science Foundation of Gansu Province (Grant No. ZS021-A25-051-N); Scientific & Technological Tackle Key Projects of Gansu Province, China. (Grant No. 2GS035-A43-048-01).
*Corresponding author. Tel./fax: 86-931-8915686