当归多糖铁治疗溶血性贫血及骨髓损伤小鼠的实验研究

当归多糖铁治疗溶血性贫血及骨髓损伤小鼠的实验研究

王珮珮, 张玉, 王凯平^*, 李珂

1. 华中科技大学同济医学院协和医院药剂科, 湖北武汉 430022;
2. 华中科技大学同济医学院药学院, 湖北武汉 430030

收稿日期:2008-03-19 修回日期:2008-08-10 出版日期:2008-09-15 发布日期:2008-09-15
通讯作者: 王凯平*

Effects of angelica sinensis polysaccharide-iron complex on hemolytic anemia and bone marrow injury in mice

Pei-Pei Wang, Yu Zhang, Kai-Ping Wang^*, Ke Li

1. Pharmaceutical Preparation Section of Union Hospital,
Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China;
2. Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China

Received:2008-03-19 Revised:2008-08-10 Online:2008-09-15 Published:2008-09-15
Contact: Kai-Ping Wang*

摘要/Abstract

摘要：

当归多糖铁是本实验室利用当归多糖和高价铁为原料合成的新型抗贫血药物。本实验主要考察当归多糖铁复合物(APIC)对溶血性贫血小鼠和骨髓损伤小鼠的治疗效果。通过腹腔注射苯肼建立溶血性贫血小鼠模型, 监测给药前后红细胞计数RBC、血红蛋白Hb、红细胞比积HCT等血液指标的变化和网织红细胞的恢复情况。用环磷酰胺(CY)和氯霉素(CH)造成小鼠骨髓损伤, 观察当归多糖铁对造模小鼠的治疗作用。溶血性贫血小鼠给予高、中剂量当归多糖铁治疗组和治疗对照药物后, 各项检测指标与模型组比较, 均有显著性差异, 且实验后Hb和HCT基本恢复至正常水平。当归多糖铁低剂量给药组的各项检测指标与模型组相比, 虽无显著性差异, 但各项指标的恢复情况要略优于模型组。当归多糖铁在贫血早期能明显促进网织红细胞的增殖和分化, 对溶血性贫血小鼠网织红细胞的恢复效果较好; 当归多糖铁能减少骨髓损伤小鼠的骨髓脂肪细胞增生, 促进其骨髓恢复。此报道在国内外尚属首次。当归多糖铁复合物具有一定的治疗溶血性贫血和减少化学药物对骨髓的损伤作用。

关键词: 当归多糖铁, 当归多糖铁, 当归多糖铁, 溶血性贫血, 溶血性贫血, 溶血性贫血, 骨髓损伤, 骨髓损伤, 骨髓损伤

Abstract: To investigate the therapeutic effects of angelica sinensis polysaccharide-iron complex (APIC) on hemolytic anemia and bone marrow injury in mice models. The hemolytic anemia mouse model was established by i.p. of phenylhydrazine (PHZ). Changes of the indices including red blood cell count (RBC), hemoglobin (Hb) and hematocrit (HCT) were determined by blood analyzer, and reticulocytes were observed by brilliant cresol blue staining during administration. Bone marrow injured mouse model was established by i.p. of cytoxan (CY) and chloramphenicol (CH), and the therapeutic effect was observed by H-E staining. The indices of APIC treated groups with the medium and high doses were higher than those of the model group significantly. Moreover, the Hb and HCT were restored to the normal level after drug treatments. In addition, APIC can promote the proliferation and differentiation of reticulocytes obviously in the early stage of anemia mice, decrease adipose cell proliferation in bone marrow of injured mice and hasten the recuperation. In conclusion, APIC has therapeutic efficacy on hemolytic anemia and bone marrow injury caused by chemicals, which is reported for the first time.

Key words: Angelica sinensis polysaccharide-iron complex, Angelica sinensis polysaccharide-iron complex, Hemolytic anemia, Hemolytic anemia, Bone marrow injury, Bone marrow injury, ,

中图分类号:

R965

Supporting: Foundation item: Foundation of Science and Technology Department of Hubei Province (Grant No. 2005AA301C04).
^*Correspondence author. Tel.: 86-27-83692745

王珮珮, 张玉, 王凯平^*, 李珂. 当归多糖铁治疗溶血性贫血及骨髓损伤小鼠的实验研究
[J]. .

Pei-Pei Wang, Yu Zhang, Kai-Ping Wang^*, Ke Li. Effects of angelica sinensis polysaccharide-iron complex on hemolytic anemia and bone marrow injury in mice
[J]. .

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