http://jcps.bjmu.edu.cn

中国药学(英文版) ›› 2017, Vol. 26 ›› Issue (8): 605-610.DOI: 10.5246/jcps.2017.08.068

• 【研究论文】 • 上一篇    下一篇

鳖甲水解多肽的纯化、鉴定及体外抗肝纤维化活性研究

胡春玲1*, 彭小芝1, 唐尹萍1, 刘焱文1, 胡祖良2, 高建蓉2   

  1. 1. 湖北中医药大学 中药资源与中药化学省级重点实验室, 湖北 武汉 430065
    2. 浙江衢化医院, 浙江 衢州 324004
  • 收稿日期:2017-05-07 修回日期:2017-06-15 出版日期:2017-08-31 发布日期:2017-07-18
  • 通讯作者: Tel.: +86-027-88920834, E-mail: 1054681196@qq.com
  • 基金资助:
    The Foundation of the Education Department of Hubei Province (Grant No. D20162004).

Purification, characterization, in vitro anti-hepatic fibrosis activity of bioactive peptides derived from Carapax Trionycis hydrolysates

Chunling Hu1*, Xiaozhi Peng1, Yinping Tang1, Yanwen Liu1, Zuliang Hu2, Jianrong Gao2   

  1. 1. Key Laboratory of Chinese Medicine Chemistry and Chinese Herbal Compound of Hubei Province, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China
    2. Zhejiang Quhua Hospital, Zhejiang, Quzhou 324004, China
  • Received:2017-05-07 Revised:2017-06-15 Online:2017-08-31 Published:2017-07-18
  • Contact: Tel.: +86-027-88920834, E-mail: 1054681196@qq.com
  • Supported by:
    The Foundation of the Education Department of Hubei Province (Grant No. D20162004).

摘要:

探讨鳖甲多肽的纯化、鉴定及其体外抗肝纤维化活性。通过超滤、葡聚糖凝胶Sephadex G-15及高效液相色谱法对鳖甲水解产物进行分离, 得到一个纯化的寡肽(CTEPH-1: Asn-Pro-Asn-Pro-Thr)。采用MTS 法和酶联免疫吸附法测定纯化多肽对活化肝星状细胞的抑制作用, 发现通过降低各型胶原蛋白和TIMP-1的含量, CTEPH-1有效的抑制了肝星状细胞的活化和增殖。因此, 该寡肽CTEPH-1可以作为功能食品的添加剂或潜在的抗肝纤维化药物。

关键词: 鳖甲多肽水解产物, 细胞外基质, 肝星状细胞

Abstract:

Anti-hepatic fibrosis peptide from Carapax Trionycis was purified, characterized, and inhibitory effect was assessed. Carapax Trionycis extract peptide hydrolysates (CTEPHs) were separated by ultrafiltration, Sephadex G-15 gel chromatography and RP-HPLC. One novel anti-hepatic fibrosis peptide (CTEPH-1: Asn-Pro-Asn-Pro-Thr) was obtained and identified. MTS assay and enzyme-linked immunosorbent assay were applied to evaluate the anti-fibrotic effect of CTEPH-1 on activated hepatic stellate cells (HSCs) in vitro. CTEPH-1 efficiently inhibited activation and proliferation of cultured HSC-T6 cells via lowering the contents of collagen and TIMP-1 except for matrix metalloproteinase-1 (MMP-1). The purified peptide might be beneficial as functional food or potential drug for treatment of liver fibrogenesis.

Key words: Carapax Trionycis extract peptide hydrolysates, Extracellular matrix, Hepatic stellate cells

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