电场方向对电穿孔技术促进咖啡因经皮渗透的影响

电场方向对电穿孔技术促进咖啡因经皮渗透的影响

胡巧红^*, 许东航

1.广东药学院, 广东广州 510006;
2.浙江大学医学院附属第二医院, 浙江杭州 310009

收稿日期:2006-01-25 修回日期:2006-05-10 出版日期:2006-06-15 发布日期:2006-06-15
通讯作者: 胡巧红*

Influence of Electric Field Direction on Enhanced Transdermal Delivery of Caffeine by Electroporation

HU Qiao-hong^*, XU Dong-hang

1.Guangdong Pharmaceutical University, Guangzhou 510006, China;
2.The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China

Received:2006-01-25 Revised:2006-05-10 Online:2006-06-15 Published:2006-06-15
Contact: HU Qiao-hong*

摘要/Abstract

摘要： 目的研究电场方向对电穿孔技术促进咖啡因经皮渗透的影响。方法应用水平双室扩散池, Ag-Ag/AgCl电极和人尸体皮肤, 进行咖啡因的经皮渗透实验, 考察电场方向对电穿孔(指数衰减型脉冲, 脉冲幅度为350 V, 脉冲率为4 pulses·min^-1, 脉冲数为25, 电容为22 μF )促渗作用的影响, 并与离子导入(0.25 mA·cm^-2, 持续4小时)进行比较。结果 1)电穿孔与离子导入均可显著增加咖啡因的经皮渗透速率和累积渗透量。2)阳极离子导入(电场方向为从供应室到接受室)的促渗作用显著大于阴极离子导入(电场方向为从接受室到供应室)的促渗作用。3)阳极电穿孔的促渗作用与阴极电穿孔的促渗作用无显著差异。4)阳极离子导入的促渗作用显著大于阳极电穿孔及阴极电穿孔的促渗作用。结论电场方向对离子导入的促渗作用有显著影响, 而对电穿孔的促渗作用没有影响。

关键词: 电穿孔, 电穿孔, 电穿孔, 电场方向, 电场方向, 电场方向, 经皮渗透, 经皮渗透, 经皮渗透, 咖啡因, 咖啡因, 咖啡因

Abstract: Aim To study the influence of electric field direction on the in vitro enhanced transdermal delivery of caffeine by electroporation. Methods Using side-by-side compartment diffusion cells method and Ag-Ag/AgCl electrodes, the transport of caffeine through human cadaver skin by electroporation (exponentially decaying pulse, pulse voltage = 350 V, pulse frequency = 4 pulses·min^-1, capacity = 22 μF, pulse length = 7 ms, 25 pulses) with different electric field directions was carried out and compared with passive diffusion and iontophoresis (0.25 mA·cm^-2, lasted for 4 h). Results (i) The cumulative quantity and flux of caffeine through human skin were increased significantly by electroporation or iontophoresis. (ii) The transport of caffeine by positive iontophoresis (with electric field from donor to receptor compartment) was significantly greater than that by negative iontophoresis (with electric field from receptor to donor compartment). (iii) The transport of caffeine by positive electroporation (with electric field from donor to receptor compartment) was similar to that by negative electroporation (with electric field from receptor to donor compartment). (iv) The enhancing effect of positive iontophoresis on the transdermal delivery of caffeine was significantly greater than that of electroporation (positive or negative). Conclusion Electric field direction significantly influences the enhancing effect of iontophoresis on the transdermal delivery of caffeine, but does not influence the enhancing effect of electroporation.

Key words: electroporation, electroporation, electric field direction, electric field direction, transdermal delivery, transdermal delivery, caffeine, caffeine

中图分类号:

943.43

Supporting: ^*Corresponding author. Tel.: 86-20-39352117; fax: 86-20-39352129

胡巧红^*, 许东航. 电场方向对电穿孔技术促进咖啡因经皮渗透的影响[J]. .

HU Qiao-hong^*, XU Dong-hang. Influence of Electric Field Direction on Enhanced Transdermal Delivery of Caffeine by Electroporation[J]. .

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