一种新型微孔底膜插入式培养皿的制作方法

一种新型微孔底膜插入式培养皿的制作方法

赵康峰, 王琪, 蒲小平^*, 杨秀伟, 朱玉真

1.兰州医学院毒理学教研室, 甘肃兰州 730000;
2.北京大学药学院细胞与分子药理学系, 北京 100083;
3.北京大学天然药物及仿生药物国家重点实验室, 北京 100083

收稿日期:2004-03-31 修回日期:2004-11-10 出版日期:2004-12-15 发布日期:2004-12-15
通讯作者: 蒲小平*

Preparation of BCEC-Astrocyte Co-culturing Membrane Plate Insert

ZHAO Kang-feng, WANG Qi, PU Xiao-ping^*, YANG Xiu-wei, ZHU Yu-zhen

1.Department of Toxicology, Lanzhou Medical College, Lanzhou 730000, China;
2.Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
3.State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, China

Received:2004-03-31 Revised:2004-11-10 Online:2004-12-15 Published:2004-12-15
Contact: PU Xiao-ping*

摘要/Abstract

摘要： 目的探讨一种新型微孔底膜插入式培养皿的制作方法, 为进行体外细胞共培养提供技术支持. 方法利用塑料离心管, 微孔滤膜为材料制备微孔底膜插入式培养皿; 在该插入式培养皿中接种大鼠脑微血管内皮细胞 (brain capillary endothelial cells, BCECs)(在星形胶质细胞条件培养液影响下), 以跨内皮细胞阻抗(trans-endothelial electronic resistance, TEER)为指标对该插入式培养皿进行评价. 结果该插入式培养皿在培养条件下(未接种细胞)15 天内未出现破损; 共培养条件下TEER增幅明显:由(66.1±13.3) Ωcm²升至(182.2±6.7) Ωcm². 结论该微孔底膜插入式培养皿制作简便, 脑微血管内皮细胞在上生长良好, 并具有可调节性和可重复使用的特点, 是一种可用于体外细胞共培养相关研究工作有效的工具.

关键词: 血脑屏障, 血脑屏障, 血脑屏障, 插入式培养皿, 插入式培养皿, 插入式培养皿, 脑毛细血管内皮细胞, 脑毛细血管内皮细胞, 脑毛细血管内皮细胞, 星形胶质细胞, 星形胶质细胞, 星形胶质细胞, 跨内皮细胞电子阻抗, 跨内皮细胞电子阻抗, 跨内皮细胞电子阻抗

Abstract: Aim To prepare a hand-made micropore membrane culture plate insert for co-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane. After seeding brain capillary endothelial cells (BCECs) on it (under the effect of astrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelial electrical resistance (TEER). Results The plate insert has a stability of at least 15 d under culture condition. TEER increased significantly under co-culture condition from (66.1±13.3) Ωcm² to (182.2±6.7) Ωcm². Conclusion This micropore membrane culture plate insert can be easily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable and recyclable. It follows that the plate insert is a useful tool for co-culture and the related research fields.

Key words: blood-brain barrier, blood-brain barrier, culture plate insert, culture plate insert, brain capillary endothelial cell, brain capillary endothelial cell, astrocyte, astrocyte, trans-endothelial electronic resistance, trans-endothelial electronic resistance

中图分类号:

R965.2

Supporting: Foundation item:National Medicine 863 Project (No. 2002AA2Z343C)
^*Corresponding author. Tel.: 86-10-82802431

赵康峰, 王琪, 蒲小平^*, 杨秀伟, 朱玉真. 一种新型微孔底膜插入式培养皿的制作方法[J]. .

ZHAO Kang-feng, WANG Qi, PU Xiao-ping^*, YANG Xiu-wei, ZHU Yu-zhen. Preparation of BCEC-Astrocyte Co-culturing Membrane Plate Insert[J]. .

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