关键词: 伊文思蓝, 脂质体, DSPE-PEG, Tween80, Brij35, 组织分布

Abstract: Aim To improve the stability and optimize the tissue distribution of Evans blue liposome in rats, some surfactants such as DSPE PEG, Tween80, and Brij35 were used to modify the Evans blue liposome. Methods The Evans blue liposome was prepared by the reverse phase evaporation method. The effect of cholesterol on the encapsulation percentage of Evans blue was studied. The effects of DSPE PEG, Tween80, and Brij35 on the encapsulation percentage and tissue distribution of Evans blue liposome in the rat were determined. Results The top encapsulation percentage of Evans blue liposome is 25.30%. After modification by DSPE PEG, Tween80, and Brij35, the encapsulation percentages decreased slightly, but not significantly. After modification, the Evans blue concentrations deceased in the liver, spleen, lung and kidney, but increased in the brain, especially in the EB-L-Tween80 group. Conclusion DSPE-PEG, Tween80, and Brij35 have slight effect on the encapsulation percentage of Evans blue liposome. The effect of Brij35 on the distribution of Evans blue liposome is similar to that of DSPE-PEG because they both is prevent the reticuloendothelial system (RES) from clearing liposome. The Evans blue concentration in the brain is greatly improved when Tween80 is used to modify the EB liposome, which is good information for preparing liposome targeting the brain.

Key words: Evans blue, Evans blue, liposomes, liposomes, DSPE-PEG, DSPE-PEG, Tween80, Tween80, Brij35, Brij35, tissue distribution, tissue distribution