用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BABL/c小鼠建立动物胃炎模型

用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BABL/c小鼠建立动物胃炎模型

阮国瑞, 史俊华, 张雪萍, 陈平圣, 尹克铮, 葛培玲, 许曼华, 许祥熹

1.东南大学医学院微生物和免疫学系, 南京 210009,
2.北京大学人民医院血研所, 北京 100044;
3.东南大学医学院病理学系, 南京 210009;
4.东南大学附属中大医院内科, 南京 210009

收稿日期:2002-02-02 修回日期:2002-04-16 出版日期:2002-06-15 发布日期:2002-06-15

Helicobacter Pylori-induced Gastritis Model in BALB/c Mice Infected With Fresh Isolates from a Human Complex Ulcer Patient

Ruan Guorui, Shi Junhua, Zhang Xueping, Chen Pingsheng, Yin Kezheng, Ge Peiling, Xu Manhua, Xu Xiangxi

1. Department of Microbiology and Immunology, Medical College of Southeast University, Nanjing 210009;
2. Institute of Hematolgy and People's Hospital, Peking University, Beijing 100044;
3. Department of Pathology, Medical College of Southeast University, Nanjing 210009;
4. Department of Internal Medicine, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009

Received:2002-02-02 Revised:2002-04-16 Online:2002-06-15 Published:2002-06-15

摘要/Abstract

摘要： 本研究的目的在于用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BABL/c小鼠建立动物胃炎模型.幽门螺杆菌(H.pylori)是由Warren和Marshall在1989年首次发现的, 此杆菌与胃炎和消化性溃疡相关联, 从而彻底改变了对胃、十二指肠疾病的认识.1990年澳大利亚国际胃肠病学术会议确认H.pylori是导致胃炎的重要病因, 由此引起的胃炎是发病率最高的消化性疾病, 但仍有许多方面了解不够或有不同认识, 存在的主要问题是缺乏简单、易控制的动物模型.我们选用复合性溃疡患者的幽门螺杆菌临床新鲜分离株, 在微需氧条件下进行培养传代, 然后按照10⁹集落形成单位/mL的菌量接种BALB/c小鼠, 接种前将小鼠禁食24小时, 并用0.25mL 10% NaHCO3稍微中和胃部酸性, 15分钟后接种, 并于第3、5天分别重复接种.于最后一次接种后5天, 2, 3, 4周分批处死动物, 取出小鼠接近幽门部的胃粘膜组织, 一部分用于H.pylori鉴定, 另一部分用于病理学检测.结果表明, 接种幽门螺杆菌的实验组小鼠胃粘膜能分离出幽门螺杆菌, 病理切片显示实验组小鼠胃粘膜有不同程度的胃炎, 而空白对照组小鼠则无明显的炎症表现.从而建立了用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BALB/c小鼠的动物胃炎模型.该模型可用于临床用药的筛选和疫苗研制.

关键词: 幽门螺杆菌, 胃炎, BABL/c小鼠

Abstract: A useful helicobacter pylori-induced gastritis model using BALB/c mice was established for mimicking of human gastritis induced by Helicobacter pylori (H. pylori). The H. pylori isolates were obtained freshly from a human complexulcer patient. BALB/c mice were fasted for 24 h and then 0.25 mL of 0.2 mol·L^-1 NaHCO3 was administered after by gavageto each mouse and 0.5 mL of 109 colonies formation unit per milliliter (CFU/mL) of H. pylori was administered 15 min. Onthe 3^rd day and 5^thday, the H. pylori inoculations were repeated. The inoculated mice were sacrificed in batch on the 5^th day, in the 2^nd week, 3^rd week and 4^th week. The gastric mucous membrane near pyloric portion was removed, treated and then cultured under microaerobic condition for detection of H.pylori. The remainders of the gastric membrane were fixed by 10% formaldehyde solution for pathological detection. The results showed that the H. pylori could be separated from the gastric membranes of inoculated mice. Obvious invasion of inflammatory cells in the gastric membranes of inoculated mice could be ob-served from pathologieal sections. It can be concluded that the inoculating fresh human H. pylori isolates can produce mousegastritis. This model of BALB/c mice can be used for evaluating the therapeutic agents for the treatment of gastritis induced by H. pylori.

Key words: Helicobacter pylori, Helicobacter pylori, Gastritis , Gastritis , BALB/c mice, BALB/c mice

Supporting:

阮国瑞, 史俊华, 张雪萍, 陈平圣, 尹克铮, 葛培玲, 许曼华, 许祥熹. 用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BABL/c小鼠建立动物胃炎模型[J]. .

Ruan Guorui, Shi Junhua, Zhang Xueping, Chen Pingsheng, Yin Kezheng, Ge Peiling, Xu Manhua, Xu Xiangxi. Helicobacter Pylori-induced Gastritis Model in BALB/c Mice Infected With Fresh Isolates from a Human Complex Ulcer Patient[J]. .