填充柱和毛细管亚临界水色谱-FID检测的研究

填充柱和毛细管亚临界水色谱-FID检测的研究

陆峰, 李玲, 孙鹏, 吴玉田

第二军医大学药学院, 上海 200433

收稿日期:2000-11-25 修回日期:2001-01-20 出版日期:2001-03-15 发布日期:2001-03-15

Sub-critical Column and Capillary Chromatography with Water as Mobile Phase and Flame Ionization Detection

Lu Feng, Li Ling, Sun Peng, Wu Yutian

College of Pharmacy, Second Military Medical University, Shanghai 200433

Received:2000-11-25 Revised:2001-01-20 Online:2001-03-15 Published:2001-03-15

摘要/Abstract

摘要： 本文采用纯水作流动相, 在一定压力下升高系统温度以保证水处在亚临界状态, FID为检测器, 分析了甲醇、异丙醇、正丁醇、2-丁醇、正戊醇、异戊醇、己醇等具有中等以上极性的醇。填充柱(1 mm i.d.) 流速50 μl·min^-1, 毛细管柱(50 μm i.d.) 流速70 μl·min^-1。升高温度至140 °C, 结合温度梯度, 亚临界水可以模拟高效液相色谱常用的甲醇-水混合流动相的极性, 可以显著缩短分析时间并改善峰形。分析系统保证了亚临界状态, 并有望应用于无紫外吸收、含量极低的极性化合物的分析。

关键词: 亚临界水色谱, FID检测, 极性化合物

Abstract: A sub-critical chromatography (SubWC) with water as mobile phase and FID detection system is employed to separate several alcohols with high or medium polarity, with pure water as the eluent. The flow rate gets up to 50 μl·min^-1 for packed column (1 mm i.d.) and 70 μl·min^-1 for capillary (50 μm i.d.). Increasing the temperature up to 140 °C, together with temperature programming, markedly improves the separation and peak shapes within short analysis time. Sub-critical state is guaranteed.

Key words: Sub-critical water chromatography, FID, Polar compounds

Supporting:

陆峰, 李玲, 孙鹏, 吴玉田. 填充柱和毛细管亚临界水色谱-FID检测的研究[J]. .

Lu Feng, Li Ling, Sun Peng, Wu Yutian. Sub-critical Column and Capillary Chromatography with Water as Mobile Phase and Flame Ionization Detection[J]. .