填充毛细管电色谱柱的制备与评价

填充毛细管电色谱柱的制备与评价

宁保明, 郑杰, 张国栋, 徐秉玖^*

北京大学药学院, 北京 100083

收稿日期:2000-10-09 修回日期:2000-12-28 出版日期:2001-03-15 发布日期:2001-03-15
通讯作者: 徐秉玖*

Preparation and Evaluation of Immobilized SE-30 Coated Stationary Phases for CEC

Ning Baoming, Zheng Jie, Zhang Guodong, Xu Bingjiu^*

School of Pharmaceutical Sciences, Peking University, Beijing 100083

Received:2000-10-09 Revised:2000-12-28 Online:2001-03-15 Published:2001-03-15
Contact: Xu Bingjiu*

摘要/Abstract

摘要： 本文报道了一种简便、快速的填充毛细管电色谱柱制备技术, 合成了SE-30 涂覆ODS的新型填料。发现这种填料既可以适合做填充毛细管电色谱柱的柱塞, 又可以作为一种固定相。考察了极性与非极性化合物在这种固定相上的色谱行为。固定相非常稳定, 在pH 值为11.7 的碱性流动相条件下可连续进样380次。

关键词: 毛细管电色谱, SE-30 涂覆 ODS, 交联

Abstract: A new type of stationary phase for capillary electrochromatography (CEC), immobilized SE-30 coated silica gel, was developed and the columns packed with this new phase were prepared and evaluated. It was found that this phase could be used to make frits for the micro-columns as well as to pack the bulk of the column. By sticking together the particles in the whole of the column bed, this new packing resulted in columns with stable performance, even under rigorous conditions: more than 380 consecutive separations were effected with these columns with the mobile phase pH of 11.7.

Key words: Capillary electrochromatography, SE-30 coated ODS stationary phase, Crosslinking

Supporting:

宁保明, 郑杰, 张国栋, 徐秉玖^*. 填充毛细管电色谱柱的制备与评价[J]. .

Ning Baoming, Zheng Jie, Zhang Guodong, Xu Bingjiu^*. Preparation and Evaluation of Immobilized SE-30 Coated Stationary Phases for CEC[J]. .