导数差示脉冲极谱法用于抗生素的定量研究

导数差示脉冲极谱法用于抗生素的定量研究

梁云爱, 王杰

1. 日照市药品检验所, 日照 276800;
2. 山东省药品检验所, 济南 250012

收稿日期:1997-11-05 修回日期:1998-03-09 出版日期:1999-06-15 发布日期:1999-06-15

Studies on Quantitative Determination of Antibiotics by the Derivative Differential Pulse Polarography

Liang Yunai, Wang Jie

1. Rizhao Institute for Drug Control. Rizhao, Shandong 276800
2. Shandong Provincial Institute for Drug Control. Jinan, Shandong 250012

Received:1997-11-05 Revised:1998-03-09 Online:1999-06-15 Published:1999-06-15

摘要/Abstract

摘要： 本文用一阶导数差示脉冲极谱法(FDDPP) 对链霉素、青霉素G、土霉素,用二阶导数差示脉冲极谱法(SDDPP)对氯霉素、卡那霉素、核糖霉素进行了定量研究。链霉素、青霉素G、土霉素、氯霉素、卡那霉素、核糖霉素分别在200~700 μg·ml^-1, 35~210 μg·ml^-1, 10~60 μg·ml^-1, 32~192 μg·ml^-1, 150~900 μg·ml^-1, 80~480 μg·ml^-1的范围内与导数差示脉冲极谱峰幅值呈极为显著的线性关系(P<0.01)。链霉素、青霉素G、土霉素、氯霉素、卡那霉素、核糖霉素的峰电位、检测限分别为0.100V, 5.6×10^-8 mol·L^-1, -1.400V, 8.0×10^-9 mol·L^-1, -1.536V, 6.9×10^-7 mol.L^-1, -0.060V, 60×10^-9 mol·L^-1, -0.370V, 4.6×10^-8 mol·L^-1 和-0.105V, 7.2×10^-9 mol·L^-1。方法简便、快速、灵敏, 结果准确。

关键词: 导数差示脉冲极谱法(DDPP), 链霉素, 青霉素, 土霉素, 氯霉素, 卡那霉素, 核糖霉素

Abstract: Quantitative determinations of streptomycin, benzylpeincillin and oxytetracycline by the first derivative differential pulse polarography (FDDPP), and of chloralnphenicol, kanamycin and ribostamycin by the second derivative differential Pulse polarography (SDDPP) were respectively studied. They showed very good linear relationship to the polarographic Peak heights (amplitudes) in the concentration ranges of 200~700 μg·ml^-1 (streptomycin), 35~210 μg·ml^-1 (benzylpenicillin), 10~60 μg·ml^-1 (oxytetracycline), 32~192 μg·ml^-1 (chloramphenicol), 150~900 μg·ml^-1 (kanamycin), and 80~480 μg·ml^-1 (ribostamycin) (p<0.01). The peak potentials and detection limits of streptomycin, benzylpenicillin, oxytetracycline, chloramphenicol, kanamycin and ribostamycin are 0. 100V, 5.6×10^-8 mol·L^-1, -1.400V, 8.0×10^-9 mol·L^-1, -1.536V, 6.9×10^-7 mol·L^-1,-0.060V, 6.0×10^-9 mol·L^-1, -0.370V, 4.6×10^-8 mol·L^-1, and -0.105V, 7.2×10^-9 mol·L^-1, respectively. This method is simple, rapid and sensitive, and the results are accurate.

Key words: Derivative differential pulse polarography(DDPP), Streptomycin, Benzylpenicillin, Oxytetracycline, Chloramphenicol, Kanamycin, Ribostamycin

Supporting:

梁云爱, 王杰. 导数差示脉冲极谱法用于抗生素的定量研究[J]. .

Liang Yunai, Wang Jie. Studies on Quantitative Determination of Antibiotics by the Derivative Differential Pulse Polarography[J]. .

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