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浙贝母种子休眠解除的低温效应

高文远, 李志亮, 肖培根   

  1. 中国医学科学院中国协和医科大学药用植物研究所, 北京 100094
  • 收稿日期:1996-05-14 修回日期:1997-03-22 出版日期:1997-09-15 发布日期:1997-09-15

Temperature Effects on Seed Dormancy Relieving of Frit-illaria thunbergii Miq.

Wen-Yuan Gao, Zhi-Liang Li, Pei-Gen Xiao   

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciencesand Peking Union Medical College, Beijing 100094
  • Received:1996-05-14 Revised:1997-03-22 Online:1997-09-15 Published:1997-09-15

摘要: 浙贝母种子的休眠在8~10 ºC下需要50天时间能够解除, 而在3~5 ºC下则需要80天时间。为了研究浙贝母种子休眠解除的生理基础, 利用分光光度法和电泳方法研究了过氧化物酶、酸性磷酸酶和蛋白质的变化。结果表明, 随着低温处理, 过氧化物酶活性和酸性磷酸酶的活性升高, 蛋白质电泳图谱上有新带出现。这些变化最终导致了休眠的解除。

关键词: 浙贝母, 休眠, 过氧化物酶, 酸性磷酸酶, 蛋白质

Abstract: The seed dormancy relieving of Fritillaria thunbergii Miq. required 50 days at 8~10 ºC and 80 days at 3~5 ºC. In order to study the physiological basis for seed dormancy relieving. peroxidase, acid phosphatase and proteins were assayed by spectrophotometric analysis and electrophoresis. The results showed that peroxidase activity and acid phosphatase activity increased and new bands were detected in protein patterns as the seeds were treated with low temperature, these changes resulted in the seed dormancy relieving.

Key words: Fritillaria thunbergii Miq, Dormancy, Pcroxidase, Acid phosphatase, Protein

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