二阶导数差示脉冲极谱法用于中草药有效成分的定量研究

二阶导数差示脉冲极谱法用于中草药有效成分的定量研究

梁云爱

日照市药品检验所, 日照, 山东 276800

收稿日期:1996-07-19 修回日期:1997-01-20 出版日期:1997-09-15 发布日期:1997-09-15

Studies on Quantitative Determination of Active Principies in Chinese Herbal Medicine by Second DerivativeDifferential Pulse Polarography

Yun-Ai Liang

Rizhao InstituteforDrug Control, Rizhao, Shandong 276800

Received:1996-07-19 Revised:1997-01-20 Online:1997-09-15 Published:1997-09-15

摘要/Abstract

摘要： 本文建立了中草药黄芩中黄芩甙、青黛中靛蓝、牡丹皮中丹皮酚、荜茇中胡椒碱的二阶导数差示脉冲极谱的定量分析方法。黄芩、靛蓝、丹皮酚和胡椒碱的化学结构中都含有, 在酸性条件下, 于滴汞电极上均可发生还原反应生成, 并分别于-1.550 V, -1.300 V, -1.630 V, -0.860 V(vs Ag/AgCl) 处出现良好的二阶导数差示脉冲极谱峰。黄芩、靛蓝、丹皮酚和胡极碱分别在4.5×10^-5~2.7×10^-4 mol·L^-1, 3.8×10^-5~2.5×10^-4 mol·L^-1, 1.0~6.0×10^-4 mol·L^-1和7.0×10^-5~2.5×10^-4 mol·L^-1。范围内, 浓度与其二阶导数差示脉冲极谱峰幅值呈极为显著的线性关系p<0.01), 检测限分别为9.0×10^-8 mol·L^-1, 8.4×10^-9 mol·L^-1, 9.2×10^-9 mol·L^-1和8.7×10^-9 mol·L^-1。方法简便、快速、灵敏, 结果准确。

关键词: 二阶导数差示脉冲极谱法, 黄芩甙, 靛蓝, 丹皮酚, 胡椒碱

Abstract: The second derivative differential pulse polarography was developed for the quantitative determination ofactive principles in medicinal herbs(baicalin in Scutellaria baicalensis Georgi, indigo in Baphicacanthus cusia(Nees) Bremek, paeonol in Paeonia suffruticosa Andr.and piperine in Piper longum L.). The group in baicalin, indigo, paeonol and piperine could be reduced to in acidic solution at the dropping mercury electrode, and showed very good second derivative differential pulse polarographic peak at -1.550 V, -1.300 V, -1.630 V and -0.860 V (Vs Ag/AgCl) respectively. The linear relationship between concentration of baicalin, indigo, paeonol, piperine and peak height was obtained in the concentration range of 4.5×10^-5~2.7×10^-4 mol·L^-1, 3.8×10^-5~2.5×10^-4 mol·L^-1, 1.0~6.0×10^-4 mol·L^-1 and 7.0×10^-5~2.5×10^-4 mol·L^-1 and the determination limits were 9.0×10^-8 mol·L^-1, 8.4×10^-9 mol·L^-1, 9.2×10^-9 mol·L^-1 and 8.7×10^-9 mol·L^-1 respectively.fhis method is simple, rapid and sensitive, and the results are accurate.

Key words: Second derivative differential pulse polarography, Baicalin, Indigo, Paeonol, Piperine

Supporting:

梁云爱. 二阶导数差示脉冲极谱法用于中草药有效成分的定量研究[J]. .

Yun-Ai Liang. Studies on Quantitative Determination of Active Principies in Chinese Herbal Medicine by Second DerivativeDifferential Pulse Polarography[J]. .

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