中药材蒲公英及其混淆品的DNA指纹鉴别研究

中药材蒲公英及其混淆品的DNA指纹鉴别研究

曹晖, 毕培曦, 邵鹏柱

1. 中国中医研究院中药研究所, 北京 100700;
2. 香港中文大学生物系, 香港;
3. 香港中文大学生物化学系, 香港;
4. 香港中文大学中药研究中心, 香港

收稿日期:1996-03-12 修回日期:1996-09-23 出版日期:1996-12-15 发布日期:1996-12-15

A Molecular Approach to Identification of the Chinese Drug“Pu Gong Ying”(Herba Taraxaci) and Six Adulterants by DNA Fingerprinting Using Random Primed PolymeraseChain Resaction (PCR)

Hui Cao, Paul Pui-Hay But, Pang-Chui Shaw

1. Institute of Chinese Materia Medica; China Academy of Traditional Chinese Medicine; Beijing 100700.
2. Department of Biology; The Chinese University of Hong Kong
3. Department of Biochemistry, The Chine University if Hong Kong;
4. Chinese Medicinal Material Research Center, The Chines University of Hong Kong

Received:1996-03-12 Revised:1996-09-23 Online:1996-12-15 Published:1996-12-15

摘要/Abstract

摘要： 采用分子生物技术包括任意引物聚合酶链式反应(AP-PCR)和随意扩增多态性DNA(RAPD)方法扩增蒲公英及其六种土公英混淆品的基因组DNA, 获得清晰可靠的DNA指纹图谱。根据琼脂糖胶上显示的DNA带型差异可鉴别蒲公英和土公英混淆品。

关键词: 中药材鉴定, 蒲公英, DNA指纹图谱, 聚合酶链式反应

Abstract: DNA fingerprinting among members of the Chinese drug Pu Gong Ying (Taraxacum mongolicum Hand, -Mazz.) and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction (PCR) including arbitrarily primed polymerase chain reaction (AP-PCR) and random amplified polymorphic DNA (RAPD). Distinctive, reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long (20 and 24 mer) and one short (10 mer) randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover, the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore, AP-PCR and RAPD methods can be used for identifying Chinese drugs.

Key words: Chinese drug identification, Taraxacum mongolicum, DNA fingerprinting, Polymerase chain reaction

Supporting:

曹晖, 毕培曦, 邵鹏柱. 中药材蒲公英及其混淆品的DNA指纹鉴别研究[J]. .

Hui Cao, Paul Pui-Hay But, Pang-Chui Shaw. A Molecular Approach to Identification of the Chinese Drug“Pu Gong Ying”(Herba Taraxaci) and Six Adulterants by DNA Fingerprinting Using Random Primed PolymeraseChain Resaction (PCR) [J]. .