木桔根甲醇提取物和其化学成分对小鼠在体和离体肝脏组织脂质过氧化的抑制作用的研究

木桔根甲醇提取物和其化学成分对小鼠在体和离体肝脏组织脂质过氧化的抑制作用的研究

杨秀伟, 服部征雄, 难波恒雄

1. 北京医科大学天然药物及仿生药物国家重点实验室, 北京 100083;
2. 日本国立富山医科药科大学和汉药研究所, 富山 930-01, 日本

收稿日期:1996-04-03 修回日期:1996-07-02 出版日期:1996-09-15 发布日期:1996-09-15

Studies on the Anti-lipid Peroxidative Actions of the Methanolic Extract of the Root of Aegle marmelos and Its Constituents In Vivo and In Vitro

Xiu-Wei Yang, Masao Hattori, Tsuneo Namba

1. National Research Laboratories of Natural and Biomimetic Drugs; Beijing Medical University; Beijing 100083;
2. Research Institute for Wakan-Yaku; Toyama Medical and Pharmaceutical University; Toyama 930-01, Japan

Received:1996-04-03 Revised:1996-07-02 Online:1996-09-15 Published:1996-09-15

摘要/Abstract

摘要： 本文报道木桔根甲醇提取物(MERA) 及其化学成分对小鼠在体和离体肝脏组织或胞液(cytoso l) 脂质过氧化的抑制作用。MERA能够明显增加肝细胞中超氧化物岐化酶和谷胱甘肽过氧化物酶的活性, 而对过氧化氢酶活性的影响无统计学意义。其主要成分之一4-甲氧基-1-甲基-2-喹诺酮(MMQ)有升高FeCl2-抗坏血酸(AA)-ADP中毒小鼠肝细胞中SOD活性的作用。从木桔根分离出的各类化合物对离体条件下FeCl2-抗坏血酸, CCl4-NADPH和ADP-NADPH等自由基发生系统诱导的大鼠肝细胞中脂质过氧化物质形成有程度不同的抑制作用。在受试化合物中, MMQ和其衍生物花椒喹诺酮(integriquinolone)对上述三种自由基发生系统引起的脂质过氧化有较强的抑制作用; 在选定的浓度下, 其作用与(-生育酚(维生素E)的效果类同。

关键词: 木桔, 抗脂质过氧化作用, 4-甲氧基-1-甲基-2-喹诺酮(MMQ), 超氧化物岐化酶, 过氧化氢酶, 谷胱甘肽过氧化物酶

Abstract: The inhibitory effect of the methanolic extract of the root of Aegle marmelos (MERA) and its constituents on the lipid peroxidation in vivo and in vitro were studied. The results suggested that MERA increased the activities of superoxide dismutase (SOD) and GSH-peroxidase in the liver cytosol of mice, but showed no significant effect on the activity of catalase, and one of its major constituents, 4-methoxy-1-methyl-2-quinolone (MMQ) increased the activity of SOD in liver tissue of mice intoxicated with FeCl2-ascorbic acid (AA)-ADP in vivo. Various constituents isolated from the root of title plant inhibited the lipid peroxidation in rat liver homogenate, which was in vitro induced by FeCl2-ascorbic acid, CCl4-NADPH, or ADP-NADPH. Of the test compounds, MMQ and its derivatives integriquinolone were similar to (-tocopherol in inhibiting MDA production in rat liver microsomes induced by Fe²⁺-ascorbate, CCl4-NADPH, or ADP-NADPH.

Key words: Aegle marmelos, Anti-lipid peroxidation, 4-Methoxy-1-methyl-2-quinolone (MMQ), Superoxide dismutase (SOD), Catalase, GSH-peroxi

Supporting:

杨秀伟, 服部征雄, 难波恒雄. 木桔根甲醇提取物和其化学成分对小鼠在体和离体肝脏组织脂质过氧化的抑制作用的研究[J]. .

Xiu-Wei Yang, Masao Hattori, Tsuneo Namba . Studies on the Anti-lipid Peroxidative Actions of the Methanolic Extract of the Root of Aegle marmelos and Its Constituents In Vivo and In Vitro[J]. .

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