奥司他韦修饰纳米金棒快速可视化检测流感病毒

doi:10.5246/jcps.2022.07.042

中国药学(英文版) ›› 2022, Vol. 31 ›› Issue (7): 491-498.DOI: 10.5246/jcps.2022.07.042

• 【研究论文】 • 下一篇

奥司他韦修饰纳米金棒快速可视化检测流感病毒

刘文彬¹, 刘东¹, 王雪¹, 杨照亮², 何筠³, 杨杨¹^,^*()

1. 天津科技大学生物工程学院, 天津 300457
2. 安序源成生物科技 (天津) 有限公司, 天津 300457
3. 安序源生物科技 (深圳) 有限公司, 广东深圳 518052

收稿日期:2021-07-30 修回日期:2021-08-10 接受日期:2021-12-14 出版日期:2022-07-31 发布日期:2022-07-31
通讯作者: 杨杨
作者简介:
+ Tel.: +86-22-60912562, E-mail: yyang@tust.edu.cn
基金资助:
Natural Science Foundation of China (Grant No. 81773583), Tackling Key Research Project for New Corona Virus Prevention and Treatment of Tianjin University of Science & Technology (Grant No. 2020STCV0019).

Oseltamivir decorated gold nanorods for visible rapid detection of influenza virus

Wenbin Liu¹, Dong Liu¹, Xue Wang¹, Zhaoliang Yang², Yun He³, Yang Yang¹^,^*()

1 College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
2 Axsyn (Tianjin) Biotechnology Co., Ltd., Tianjin 300457, China
3 Anxuyuan Biotechnology (Shenzhen) Co., Ltd., Shenzhen 518052, Guangdong, China

Received:2021-07-30 Revised:2021-08-10 Accepted:2021-12-14 Online:2022-07-31 Published:2022-07-31
Contact: Yang Yang

摘要/Abstract

摘要：

利用纳米金棒表面等离子共振吸收峰位置与强度随纳米粒子间距离变化的光学特性, 使用与流感病毒表面神经氨酸酶具有强结合的抗流感一线药物奥司他韦的硫辛酸衍生物对纳米金棒进行修饰, 借助其与流感病毒孵育前后金纳米颗粒聚集导致的颜色变化, 使用肉眼及可见分光光度计对流感病毒进行快速可视化检测。结果表明, 其检测限与现有的基于流感病毒核蛋白抗原-抗体相互作用的胶体金试纸条检测限相当, 其廉价稳定的特性, 具备进一步发展为新型流感检测试剂盒的潜力。

关键词: 纳米金棒, 奥司他韦, 流感病毒, 神经氨酸酶, 可视化检测

Abstract:

In the present study, we developed a novel colorimetric strategy for the visible rapid detection of the influenza virus based on the unique optical properties of gold nanorods. Specifically, lipoic acid-modified Oseltamivir (OS), which is a strong neuraminidase inhibitor and used as the first-line drug in the treatment of influenza, was synthesized and further attached to the surface of gold nanorods (OS-LA-GNRs). The absorption band and density of OS-LA-GNRs were changed with the decreasing distance between the nanoparticles induced by the target-specific aggregation via strong neuraminidase–OS binding. All of these could be visible with the naked eyes and measured by UV-visible spectrophotometry. The results showed that our system had a comparable limit of detection (LOD) to the commercial colloidal gold stripes, making it feasible for wide diagnostic applications.

Key words: Gold nanorods, Oseltamivir, Influenza virus, Neuraminidase, Visible detection

Supporting:

刘文彬, 刘东, 王雪, 杨照亮, 何筠, 杨杨. 奥司他韦修饰纳米金棒快速可视化检测流感病毒[J]. 中国药学(英文版), 2022, 31(7): 491-498.

Wenbin Liu, Dong Liu, Xue Wang, Zhaoliang Yang, Yun He, Yang Yang. Oseltamivir decorated gold nanorods for visible rapid detection of influenza virus[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(7): 491-498.

图/表 3

Scheme 1. Preparation of OS decorated gold nanorods (OS-LA-GNRs). Reagents and conditions: (a) (i) (Boc)2O, Et3N, MeOH; (ii) NaOH, 95% over two steps; (b) EDCI, THF, 40%; (c) CH2Cl2, TFA, rt, 2 h, 60%.

Figure 1. Characterization of OS-LA-GNRs. (a) SEM image, (b) UV absorption spectra.

Figure 2. Detection of influenza virus using OS-LA-GNRs. (a) LSPR peak absorption ratios, where A and Ac are the LSPR peak absorption of the OS-LA-GNRs solution added analyte and OS-LA-GNRs solution, respectively; (b) colorimetric results of the virus OS-LA-GNRs mixtures; (c) LSPR peak intensity ratio (A/Ac) versus influenza virus concentration; (d) UV-Vis absorption spectra of OS-LA-GNRs incubated with influenza virus.

参考文献 21

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奥司他韦修饰纳米金棒快速可视化检测流感病毒

Oseltamivir decorated gold nanorods for visible rapid detection of influenza virus

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