米曲霉和团青霉固态发酵提高火棘果抗氧化活性、总酚和总黄酮含量

doi:10.5246/jcps.2022.06.039

中国药学(英文版) ›› 2022, Vol. 31 ›› Issue (6): 452-460.DOI: 10.5246/jcps.2022.06.039

米曲霉和团青霉固态发酵提高火棘果抗氧化活性、总酚和总黄酮含量

董建伟^*(), 李雪娇^*(), 杨晨, 张艳清, 周慧芳, 李亚丽

曲靖师范学院化学与环境科学学院, 云南曲靖 655011

收稿日期:2022-01-03 修回日期:2022-02-18 接受日期:2022-03-07 出版日期:2022-06-30 发布日期:2022-06-30
通讯作者: 董建伟, 李雪娇
作者简介:
+ Tel.: +86-874-8998658, E-mail: jwdongyn@mail.qjnu.edu.cn
+ E-mail: lixj93@outlook.com
基金资助:
Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities (Grant No. 2019FH001-109); Undergraduates Innovative Experiment Project from MOE (Grant Nos. 202010684016, 202110684004).

The antioxidant activity and total phenolic and total flavonoid contents of Pyracantha fortuneana fruit can be improved by solid-state fermentation with Rhizopus oryzae and Penicillium commune

Jianwei Dong^*(), Xuejiao Li^*(), Chen Yang, Yanqing Zhang, Huifang Zhou, Yali Li

College of Chemistry and Environmental Science, Qujing Normal University, Qujing 655011, China

Received:2022-01-03 Revised:2022-02-18 Accepted:2022-03-07 Online:2022-06-30 Published:2022-06-30
Contact: Jianwei Dong, Xuejiao Li

摘要/Abstract

摘要：

肿瘤转移是一个多步骤的复杂过程, 具有明显的随机性。本研究旨在建立一种随机数学模型来描述肿瘤转移的随机过程, 预测新化合物QAP14对小鼠肿瘤转移的抑制作用。4T1细胞植入BALB/c小鼠22天后, 收集QAP14治疗组与对照组的肺转移数据, 基于原发肿瘤和转移灶指数生长的特点和以上数据, 建立转移大小和数量的联合分布模型, 进而模拟疾病进展和QAP14的临床前药效。参数M和m分别代表转移灶体积的最大值和最小值, 估计值分别为3.24和0.0184 mm³, 转移生长速率γ和促转移时间ρ的估计值分别为0.0216/天和7.8天并在药效模型中固定, QAP14对促转移时间和转移生长速率常数的作用以指数的形式加入到模型中, 药效参数Effect_ρ和Effect_γ的估计值分别为16.6和0.327 g/mg。本研究系统描述了肿瘤转移的疾病进程以及QAP14抑制4T1乳腺癌小鼠肺转移的药效, 为临床上建立肿瘤转移的群体模型提供了有价值的参考。

关键词: 随机群体模型, PD模型, 高通量4T1, 肺转移, 乳腺癌

Abstract:

Cancer metastasis is a process with multi-step complexity and apparent randomness. In this study, we aimed to establish a stochastic mathematical model to describe the random process of cancer metastasis and predict the drug effect of QAP14 on metastasis in a mouse model. The data of lung metastases on the 22^nd day after cancer cell implantation with or without the treatment of QAP14, a new chemical compound, were collected in 4T1 breast cancer BALB/c mice. Based on the exponential growth of the primary tumor and metastatic loci, a joint distribution model of metastasis size and number was developed. Disease progression of metastasis and preclinical efficacy of QAP14 were modeled. Parameters M and m representing maximum and minimum of metastasis volume were 3.24 and 0.0184 mm³, respectively. The metastasis growth rate γ and metastasis promotion time ρ were estimated and fixed to be 0.0216 d^–1 and 7.8 d, respectively. The efficacy of QAP14 acted on metastasis promotion time and metastasis growth rate constant in an exponential term, and the effect parameter Effect_ρ and Effect_γ were 16.6 and 0.327 g/mg, respectively. In the present study, we comprehensively characterized the random process of lung metastasis and efficacy of QAP14 in 4T1 breast cancer mice, which might provide a useful reference for the establishment of a clinical population model of cancer metastasis.

Key words: Stochastic population model, PD model, 4T1, Lung metastasis, Breast cancer

Supporting:

董建伟, 李雪娇, 杨晨, 张艳清, 周慧芳, 李亚丽. 米曲霉和团青霉固态发酵提高火棘果抗氧化活性、总酚和总黄酮含量[J]. 中国药学(英文版), 2022, 31(6): 452-460.

Jianwei Dong, Xuejiao Li, Chen Yang, Yanqing Zhang, Huifang Zhou, Yali Li. The antioxidant activity and total phenolic and total flavonoid contents of Pyracantha fortuneana fruit can be improved by solid-state fermentation with Rhizopus oryzae and Penicillium commune[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(6): 452-460.

图/表 4

Figure 1. DPPH (A) and ABTS (B) radical scavenging activities, and FRAP (C) of non-fermented and fermented P. fortuneana fruits at different concentrations. Each value represents mean ± SD. Capital and low-case letters are significantly different from each other at P < 0.05 and P < 0.01, respectively.

Figure 2. TPCs (A) and TFCs (B) of non-fermented and fermented P. fortuneana fruits. Each value represents mean ± SD. Capital and low-case letters are significantly different at P < 0.05 and P < 0.01, respectively.

Table 1. Correlation analyses*.

Figure 3. 1H NMR spectra of NFPF (A), BFPF (B), ROPF (C), PCPF (D), and PSPF (E) in DMSO-d6 (400 MHz).

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米曲霉和团青霉固态发酵提高火棘果抗氧化活性、总酚和总黄酮含量

The antioxidant activity and total phenolic and total flavonoid contents of Pyracantha fortuneana fruit can be improved by solid-state fermentation with Rhizopus oryzae and Penicillium commune

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