生地黄和熟地黄中5-羟甲基糠醛的含量与色泽的相关性研究

doi:10.5246/jcps.2020.05.029

中国药学(英文版) ›› 2020, Vol. 29 ›› Issue (5): 314-321.DOI: 10.5246/jcps.2020.05.029

生地黄和熟地黄中5-羟甲基糠醛的含量与色泽的相关性研究

吴翠^1,2, 徐靓^1,2, 徐博^1,2, 李卓俊^1,2, 巢志茂^1,2*

1. 中国中医科学院中药研究所, 北京 100700
2. 国家中药材产业技术体系贮藏与包装岗位, 北京 100700

收稿日期:2019-08-22 修回日期:2019-11-13 出版日期:2020-05-31 发布日期:2020-01-05
通讯作者: Tel.: +86-13522705161, E-mail: chaozhimao@163.com
基金资助:
China Agriculture Research System (Grant No. CARS-21).

Correlation between 5-hydroxymethylfurfural content and color of Rehmanniae Radix and Rehmanniae Radix Praeparata

Cui Wu^1,2, Liang Xu^1,2, Bo Xu^1,2, Zhuojun Li^1,2, Zhimao Chao^1,2*

1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Storage & Packaging Position, Chinese Materia Medica, China Agriculture Research System, Beijing 100700, China

Received:2019-08-22 Revised:2019-11-13 Online:2020-05-31 Published:2020-01-05
Contact: Tel.: +86-13522705161, E-mail: chaozhimao@163.com
Supported by:
China Agriculture Research System (Grant No. CARS-21).

摘要/Abstract

摘要：

本研究探讨了生地黄和熟地黄中5-羟甲基糠醛(5-HMF)含量与其色泽的相关性。自然光下人眼观察色泽; 色差仪测定色度值和色差值; 烘干法测定水分; 高效液相色谱法测定5-HMF的含量; 双变量相关性分析方法探讨色泽数据与5-HMF含量的相关性。结果显示生地黄呈棕褐或深褐色, 熟地黄呈黑色。熟地黄色泽的加深体现在色度值的变化上,表现为L^*、a^*和b^*值的显著降低。各地黄样品中的水分含量均符合药典规定。5-HMF的含量在生地黄和熟地黄中分别为0.9711–25.71 μg/g和213.4–4010 μg/g, 平均含量分别为8.059 μg/g和1433 μg/g。熟地黄中5-HMF的含量显著高于生地黄。生、熟地黄中5-HMF的含量与L^*、a^*和b^*值具有显著相关性。熟地黄的色泽较生地黄深。5-HMF的含量在生地黄中低于0.02%, 而熟地黄中的含量高于0.02%, 可作为生、熟地黄判断的标志性成分。

关键词: 生地黄, 熟地黄, 色泽, 5-羟甲基糠醛, 双变量相关性分析

Abstract:

In the present study, we explored the correlation between 5-hydroxymethylfurfural (5-HMF) and color of Rehmanniae Radix (RR) and Rehmanniae Radix Praeparata (RRP). The color was observed by human eyes under sunlight. The chromatic value and color difference were detected by a colorimeter. The water content was determined by an oven-drying method. The 5-HMF content was determined by an HPLC method. The correlation between the 5-HMF content and the color was analyzed by bivariatecorrelation analysis. The results showed that the color was greyish-brown and dark brown for RR, and it was black for RRP. The deepening color of RRP was reflected in the decrease of L^*, a^* and b^* values. The water content was in accord with the requirement of Chinese Pharmacopoeia. The 5-HMF contents of RR and RRP were 0.9711–25.71 µg/g and 213.4–4010 µg/g, respectively. The average 5-HMF contents of RR and RRP were 8.059 µg/g and 1433 µg/g, respectively. The 5-HMF content in RRP was higher than that in RR. The correlations between 5-HMF content and L^*, a^* and b^* values were significant. The color of RRP was deeper than that of RR. The 5-HMF content of RRP was higher than 0.02%, while it was less than 0.02% of RR, which could be used as a marker component for judging RR and RRP.

Key words: Rehmanniae Radix, Rehmanniae Radix Praeparata, Color, 5-Hydroxymethylfurfural, Bivariate correlation analysis

中图分类号:

R284

Supporting:

吴翠, 徐靓, 徐博, 李卓俊, 巢志茂. 生地黄和熟地黄中5-羟甲基糠醛的含量与色泽的相关性研究[J]. 中国药学(英文版), 2020, 29(5): 314-321.

Cui Wu, Liang Xu, Bo Xu, Zhuojun Li, Zhimao Chao. Correlation between 5-hydroxymethylfurfural content and color of Rehmanniae Radix and Rehmanniae Radix Praeparata[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(5): 314-321.

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生地黄和熟地黄中5-羟甲基糠醛的含量与色泽的相关性研究

Correlation between 5-hydroxymethylfurfural content and color of Rehmanniae Radix and Rehmanniae Radix Praeparata

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