沙棘籽油纳米乳的制备及经皮渗透的可视化分析研究

doi:10.5246/jcps.2019.08.056

中国药学(英文版) ›› 2019, Vol. 28 ›› Issue (8): 579-594.DOI: 10.5246/jcps.2019.08.056

• 【研究论文】 • 上一篇

沙棘籽油纳米乳的制备及经皮渗透的可视化分析研究

沈琪, 王梅^*, 唐小慧, 木巴拉克.伊明江

新疆医科大学药学院物理化学与药剂学教研室, 新疆乌鲁木齐 830011

收稿日期:2019-04-08 修回日期:2019-05-23 出版日期:2019-09-02 发布日期:2019-06-12
通讯作者: Tel.: +86-0993-4362442, E-mail: wm630@163.com
基金资助:
National Natural Science Foundation of China (Grant No. 81460539) and the excellent postgraduate project in Xinjiang Medical University (Grant No. CXCY096).

Preparation of a Hippophae rhamnoides Linn oil nanoemulsion and the visualization of its transdermal permeation

Qi Shen, Mei Wang^*, Xiaohui Tang, Munire Kuerban

Physical Chemistry and Pharmacy Teaching and Research Department, College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China

Received:2019-04-08 Revised:2019-05-23 Online:2019-09-02 Published:2019-06-12
Contact: Tel.: +86-0993-4362442, E-mail: wm630@163.com
Supported by:
National Natural Science Foundation of China (Grant No. 81460539) and the excellent postgraduate project in Xinjiang Medical University (Grant No. CXCY096).

摘要/Abstract

摘要：

制备沙棘油纳米乳并对其理化性质和经皮渗透能力、皮肤刺激性进行考察。沙棘油纳米乳的最优处方为:以聚氧乙烯蓖麻油为乳化剂,二乙二醇单乙基醚为助乳化剂,肉豆蔻酸异丙酯为油相,肉豆蔻酸异丙酯:聚氧乙烯蓖麻油:沙棘油= 5%:16%:20%, Km = 4:1。制得外观澄清透明的淡黄色O/W型纳米乳,平均粒径为(52.2±4.8)nm,在透射镜下分布均一。经高速离心后,沙棘油纳米乳稳定性良好。测得沙棘油纳米乳的平均载药量为0.24 mg·mL^–¹(RSD=1.47%), 达10.68%。在激光共聚焦显微镜下, OB荧光标记的沙棘油纳米乳、纳米乳凝胶和肉豆蔻酸异丙酯溶液,透皮2小时后在表皮、真皮浅层和深层以及毛囊处的荧光强度均强于OB荧光标记的沙棘油和沙棘油乳膏。皮肤刺激性实验可知,制得的沙棘油纳米乳凝胶和沙棘油纳米乳在家兔正常和破损皮肤上均无明显可见的红肿现象。以上实验结果表明沙棘油纳米乳是一种潜在的用于皮肤治疗的传递系统。

关键词: 沙棘油, 纳米乳, 共聚焦电子显微镜, 相图

Abstract:

A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermalcharacteristics were investigated. Then its skin irritation was investigated.The optimum Hippophae rhamnoides Linn oil nanoemulsion prescription was determined using Cremophor EL as an emulsifier, Transcutol P as a co-emulsifier, and isopropyl myristate (IPM) as the oil phase (IPM%:Cremophor EL%:Hippophae rhamnoides Linn oil% = 5:16:20, Km = 4:1). The prepared Hippophaerhamnoides Linn oil nanoemulsion was pale yellow and transparent. Globular droplets were observed under a transmission electronmicroscope (TEM). The average particle size was 52.2±4.8 nm. The loading capacity was 10.68%, and the oil-in-water (O/W) type nanoemulsion was stable after centrifugation. The CLSM results showed that the fluorescence intensity of the OB Hippophae rhamnoides Linn oil nanoemulsion group was stronger than that of the Hippophae rhamnoides Linn oil cream and Hippophae rhamnoides Linn oil groups in each layer of skin. The study also showed that skin surface treated with the Hippophae rhamnoides Linn oil nanoemulsion had strong fluorescence. The skin depth had weak fluorescence, while the areas near the hair follicle and its appendages had the strongest fluorescence. The skin irritation test showed that the prepared Hippophae rhamnoides Linn oil nanoemulsion was applied to the normal and damaged skin of the rabbit, and there was no apparent redness and swelling. These results suggested that the nanoemulsion coated with Hippophae rhamnoides Linn oil was a potential delivery system for skin disorders.

Key words: Hippophae rhamnoides Linn oil, Nanoemulsion, Confocal laser scanning microscopy, Phase diagram

中图分类号:

R943

Supporting:

沈琪, 王梅, 唐小慧, 木巴拉克.伊明江. 沙棘籽油纳米乳的制备及经皮渗透的可视化分析研究[J]. 中国药学(英文版), 2019, 28(8): 579-594.

Qi Shen, Mei Wang, Xiaohui Tang, Munire Kuerban. Preparation of a Hippophae rhamnoides Linn oil nanoemulsion and the visualization of its transdermal permeation[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(8): 579-594.

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沙棘籽油纳米乳的制备及经皮渗透的可视化分析研究

Preparation of a Hippophae rhamnoides Linn oil nanoemulsion and the visualization of its transdermal permeation

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