Effect of Curcuma longa L. extract on the AP1 expression in rat cochlear fibroblasts under noise conditions

doi:10.5246/jcps.2016.09.077

中国药学(英文版) ›› 2016, Vol. 25 ›› Issue (9): 690-694.DOI: 10.5246/jcps.2016.09.077

Effect of Curcuma longa L. extract on the AP1 expression in rat cochlear fibroblasts under noise conditions

Tengku Siti Hajar Haryuna^1*, Ramsi Lutan¹, Faathir Agung Ainul Taufika¹, Ratna Anggraeni², Tengku Siti Harilza Zubaidah³

1. Department of Otorhinolaryngology, Faculty of Medicine, University of North Sumatera, Medan 20155, Indonesia
2. Department of Otorhinolaryngology, Faculty of Medicine, Padjadjaran University, Bandung 45363, Indonesia
3. Department of Ophthalmology, Faculty of Medicine, University of North Sumatera, Medan 20155, Indonesia

收稿日期:2016-03-30 修回日期:2016-04-25 出版日期:2016-09-28 发布日期:2016-05-15
通讯作者: Tel.: +628126061694, +6282167578676, E-mail: tengkusitihajarharyuna@gmail.com
基金资助:
Daftar Isian Pelaksanaan Anggaran (DIPA) 2014 (Grant No. 4806/UN5.1.R/KEU/2014) Universitas Sumatera Utara.

Effect of Curcuma longa L. extract on the AP1 expression in rat cochlear fibroblasts under noise conditions

Tengku Siti Hajar Haryuna^1*, Ramsi Lutan¹, Faathir Agung Ainul Taufika¹, Ratna Anggraeni², Tengku Siti Harilza Zubaidah³

1. Department of Otorhinolaryngology, Faculty of Medicine, University of North Sumatera, Medan 20155, Indonesia
2. Department of Otorhinolaryngology, Faculty of Medicine, Padjadjaran University, Bandung 45363, Indonesia
3. Department of Ophthalmology, Faculty of Medicine, University of North Sumatera, Medan 20155, Indonesia

Received:2016-03-30 Revised:2016-04-25 Online:2016-09-28 Published:2016-05-15
Contact: Tel.: +628126061694, +6282167578676, E-mail: tengkusitihajarharyuna@gmail.com
Supported by:
Daftar Isian Pelaksanaan Anggaran (DIPA) 2014 (Grant No. 4806/UN5.1.R/KEU/2014) Universitas Sumatera Utara.

摘要/Abstract

摘要：

Noise-induced cellular stress can cause damage to fibroblasts within the cochlear supporting tissues and lateral wall. In the present study, we aimed to evaluate the role of curcumin as the safe and effective therapeutic agent in the prevention and treatment of this condition according to the expression of activator protein-1 (AP1). A total of 24 Rattus norvegicus were randomly divided into four groups (n = 6). Group 1: control; group 2: noise (+); group 3: noise (+), 50 mg/day curcumin (+); group 4: noise (+), 100 mg/day curcumin (+). All groups (except for group 1) were subjected to a sound pressure level (SPL) of 100 dB for 2 h/day during 2 weeks. Curcumin used in this study was derived from Curcuma longa L. (Turmeric), and it was orally administered for 2 weeks. All samples were immunohistochemistrically examined for the expression of AP1 in cochlear fibroblasts. The results showed that there were significant differences for the AP1 expression (P<0.05) among all groups, except for between groups 1 and 3, or between groups 1 and 4. Our data proved that curcumin was potentially effective in the prevention and treatment of damage of fibroblasts within the cochlear supporting tissues and lateral wall due to the decreased AP1 expression following noise exposure.

关键词: Noise, Curcumin, AP1, Fibroblast, Cochlea

Abstract:

Key words: Noise, Curcumin, AP1, Fibroblast, Cochlea

中图分类号:

R961

Supporting:

Tengku Siti Hajar Haryuna, Ramsi Lutan, Faathir Agung Ainul Taufika, Ratna Anggraeni, Tengku Siti Harilza Zubaidah. Effect of Curcuma longa L. extract on the AP1 expression in rat cochlear fibroblasts under noise conditions[J]. 中国药学(英文版), 2016, 25(9): 690-694.

参考文献

[1] Man-Son-Hing, M.; Laupacis, A.; O'Connor, A.; Wells, G.; Lemelin, J.; Wood, W.; Dermer, M. Arch Intern. Med. 1996, 156, 1841-1848.
[2] Bounda, G.A.; Ngarambe, C.; Ge, W.H.; Yu, F. J. Chin. Pharm. Sci. 2013, 22, 95-100.
[3] Wadelius, M.; Chen, L.Y.; Eriksson, N.; Bumpstead, S.; Ghori, J.; Wadelius, C.; Bentley, D.; McGinnis, R.; Deloukas, P. Hum Genet. 2007, 121, 23-34.
[4] Zhang, Y.T.; Zheng, Z.; Liang, X.; Hu, X.; Li, K.X. J. Chin. Pharm. Sci. 2014, 23, 190-193.
[5] Miao, L.; Yang, J.; Huang, C.; Shen, Z. Eur. J. Clin. Pharmacol. 2007, 63, 1135-1141.
[6] Limdi, N.A.; McGwin, G.; Goldstein, J.A.; Beasley, T.M.; Arnett, D.K.; Adler, B.K.; Baird, M.F.; Acton, R.T. Clin. Pharmacol. Ther. 2008, 83, 312-321.
[7] Sconce, E.A.; Khan, T.I.; Wynne, H.A.; Avery, P.; Monkhouse, L.; King, B.P.; Wood, P.; Kesteven, P.; Daly, A.K.; Kamali, F. Blood. 2005, 106, 2329-2323.
[8] Teichert, M.; Eijgelsheim, M.; Rivadeneira, F.; Uitterlinden, A.G.; van Schaik, R.H.; Hofman, A.; De Smet, P.A.; van Gelder, T.; Visser, L.E.; Stricker, B.H.A. Hum Mol. Genet. 2009, 18, 3758-3768.
[9] Cooper, G.M.; Johnson, J.A.; Langaee, T.Y.; Feng, H.; Stanaway, I.B.; Schwarz, U.I.; Ritchie, M.D.; Stein, C.M.; Roden, D.M.; Smith, J.D. Blood. 2008, 112, 1022-1027.
[10] Rieder, M.J.; Reiner, A.P.; Rettie, A.E. J. Thromb. Haemost. 2007, 5, 2227-2234.
[11] Aquilante, C.L.; Langaee, T.Y.; Lopez, L.M. Clin. Pharmacol. Ther. 2006, 79, 291-302.
[12] Ufer, M. Clin. Pharmacokinet. 2005, 44, 1227-1246.
[13] Tadros, R.; Shakib, S. Aust. Fam Physician. 2010, 39, 476-479.
[14] Schulman, S.; Kearon, C. J. Thromb. Haemost. 2005, 3, 692-694.
[15] Deng, L.; Huang, R.; Chen, Z.; Wu, L.; Xu, D.L. Am. J. Hypertens. 2009, 22, 656-662.
[16] He, Z.; Li, Z.; Shi, Y.; Tang, W.; Huang, K.; Ma, G.; Zhou, J.; Meng, J.; Li, H.; Feng ,G.; He, L. Schizophr Res. 2007, 94, 359-365.
[17] Yang, J.Q.; Morin, S.; Verstuyft, C.; Fan, L.A.; Zhang, Y.; Xu,C.D.; Barbu, V.; Funck-Brentano, C.; Jaillon, P.; Becquemont, L. Fundam Clin. Pharmacol. 2003, 17, 373-376.
[18] Teichert, M.; Eijgelsheim, M.; Rivadeneira, F.; Uitterlinden, A.G.; van, Schaik, R.H.; Hofman, A.; De Smet, P.A.; van Gelder, T.; Visser, L.E.; Stricker B.H. Hum. Mol. Genet. 2009, 18, 3758-3768.
[19] Rieder, M.J.; Reiner, A.P.; Gage, B.F.; Nickerson, D.A.; Eby, C.S.; McLeod, H.L.; Blough, D.K.; Thummel, K.E.; Veenstra, D.L; Rettie, A.E. N. Engl. J. Med. 2005, 352, 2285-2293.
[20] Douketis, J.D.; Melo, M.; Bell, C.M.; Mamdani, M.M. Am. J. Med. 2007, 120, 369.e9-369.e14.
[21] Andersson, M.L.; Eliasson, E.; Lindh, J.D. Pharmacogenomics. 2012, 13, 757-762.

Effect of Curcuma longa L. extract on the AP1 expression in rat cochlear fibroblasts under noise conditions

Effect of Curcuma longa L. extract on the AP1 expression in rat cochlear fibroblasts under noise conditions

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	Tengku Siti Hajar Haryuna, Agustinus Hamonangan Winston Purba, Farhat Farhat, Soehartono Taat Putra. The effect of curcumin as an antioxidant on cochlea fibroblasts in ototoxic rat models[J]. 中国药学(英文版), 2018, 27(12): 847-854.
[2]	Sri Agus Sudjarwo, Koerniasari Eraiko, Giftania Wardani Sudjarwo, Koerniasari. Antioxidant activity of curcumine as protector on methylmercury induced-pancreas damage in mice[J]. 中国药学(英文版), 2017, 26(3): 196-201.
[3]	Francisco Fuentes, Yury Gomez, Ximena Paredes-Gonzalez, Avantika Barve, Sujit Nair, Siwang Yu, Constance Lay Lay Saw, Ah-Ng Tony Kong. Nrf2-mediated antioxidant and detoxifying enzyme induction by a combination of curcumin and sulforaphane[J]. 中国药学(英文版), 2016, 25(8): 559-569.