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中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (5): 335-337.DOI: 10.5246/jcps.2014.05.047

• 【简报】 • 上一篇    下一篇

重组酶在胰岛素生产中的应用

冷春生*, 崔航, 李淑楠   

  1. 通化东宝药业股份有限公司, 吉林 通化 134001
  • 收稿日期:2014-01-06 修回日期:2014-01-22 出版日期:2014-05-23 发布日期:2014-02-23
  • 通讯作者: Tel.: +86-435-5007801; +86-435-5088323; E-mail: csleng@126.com

Application of recombinant enzymes in the production of human insulin

Chunsheng Leng*, Hang Cui, Shunan Li   

  1. Tonghua Dongbao Pharmaceutical Co. Ltd., Jilin 134001, China
  • Received:2014-01-06 Revised:2014-01-22 Online:2014-05-23 Published:2014-02-23
  • Contact: Tel.: +86-435-5007801; +86-435-5088323; E-mail: csleng@126.com

摘要:

本文分别测定了重组的与天然的牛胰蛋白酶和羧肽酶B对前胰岛素融合蛋白的裂解活性。水解产物采用HPLC进行分析, 发现重组酶具有更强的活性并且副产物少, 因此重组酶在胰岛素生产中比天然的牛胰蛋白酶和羧肽酶B具有更好的前景。

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关键词: 重组胰蛋白酶, 人胰岛素, 重组羧肽酶B, 重组酶, 重组人胰岛素

Abstract:

In the present study, the cleavage activities of both recombinant and bovine enzymes(trypsin and carboxy peptidase B) were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPLC.We found that recombinant enzymes had stronger activities and produced fewer by-products. In addition, recombinant enzymes were more efficient in the production of human insulin than bovine enzymes.

Key words: Recombinant trypsin, Human insulin, Recombinant carboxy peptidase B, Recombinant enzymes, Recombinant human insulin

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Supporting: null