HPLC fingerprint-oriented preparative separation of major flavonoids from safflower extract by preparative pressurized liquid chromatography

doi:10.5246/jcps.2014.07.064

Abstract

Abstract:

The aim of this study was to rapidly isolate the major effective flavanoids from the extract of safflower (Carthamus tinctorius) using ODSmedium pressure liquidchromatography (MPLC) and semi-preparative HPLC, guided by a developed fingerprint. Twelve compounds were isolated and their structures were elucidated as kaempferol 3-O-β-D-rutinoside (1), kaempferol 3-O-β-D-glucoside (2), rutin (3),quercetin 3-O-β-D-glucoside (4), 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside (5), 6-hydroxykaempferol 3-O-β-D-glucoside (6), 6-hydroxykaempferol 6,7-di-O-β-D-glucoside (7), 6-hydroxykaempferol 3-O-β-D-rutinoside (8), 6-hydroxykaempferol 3,6-di-O-β-D-glucosyl 7-O-β-D-glucuronide (9), isosafflomin C (10), safflomin C (11) and hydroxysafflor yellow A (12) by spectroscopic analysis and comparing with the literature. Our results demonstratedthat preparative pressurized liquid chromatography combined with HPLC fingerprint guide is an efficient tool to isolate the target compounds quickly.

Key words: Extract of Carthamus tinctorius, Flavonoids, Isolation, Preparative pressurized liquid chromatography, HPLC fingerprint

CLC Number:

R931

Supporting:

Jinfeng Chen, Pengfei Tu, Yong Jiang. HPLC fingerprint-oriented preparative separation of major flavonoids from safflower extract by preparative pressurized liquid chromatography[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(7): 490-495.

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