Abstract: To investigate the chemical constituents of the stems of Aquilaria sinensis (Lour.) Gilg, the separation and purification were performed by solvent extraction, repeated chromatography on silica gel, Sephadex LH-20 and prep-HPLC. The structures were determined by spectrum analysis. Thirteen known compounds were isolated and their structures were identified as justicidin A (1), justidin F (2), ciwujiatone (3), (+) syringaresinol (4), syringaresinol-4,4'-di-O-β-D-glucopyranoside (5), syringaresinol-4'-O-β-D-glucopyranoside (6), curuilignan D (7), syringin (8), koaburaside (9), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside (10), 3,4,5-trimethoxyphenyl-1-O-β-D-apiofuranosyl-(1''→6') glucopyranoside (11), 7-ketositosterol (12), 7-oxo-5,6-dihydrostigmasterol (13). All the compounds described above were isolated from genus Aquilaria for the first time.

Key words: Aquilaria sinensis, Thymelaeaceae, Chemical constituents, Lignans, Phenolicglycosides